Rabbit anti-Human BHLHB5 Polyclonal Antibody | anti-BHLHB5 antibody

BHLHB5, ID (BHLHE22, BHLHB5, TNRC20, Class E basic helix-loop-helix protein 22, Class B basic helix-loop-helix protein 5, Trinucleotide repeat-containing gene 20 protein) (Azide free) (HRP)

Cat. Num. AAA6278366

• Gene Names: BHLHE22; Beta3; BHLHB5; Beta3a; CAGL85; TNRC20
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BHLHB5; Polyclonal Antibody; ID (BHLHE22; TNRC20; Class E basic helix-loop-helix protein 22; Class B basic helix-loop-helix protein 5; Trinucleotide repeat-containing gene 20 protein) (Azide free) (HRP); BHLHE22; Trinucleotide repeat-containing gene 20 protein; anti-BHLHB5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-BHLHB5 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: BHLHB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243~272 amino acids from the Central region of human BHLHB5.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of BHLHB5 Antibody (Center) in HL-60 cell line lysates (35ug/lane). BHLHB5 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(BHLHB5 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BHLHB5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(BHLHB5 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-BHLHB5 antibody

BHLHB5 inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. BHLHB5 despite the presence of an intact basic domain, does not bind to DNA.

Product Categories/Family for anti-BHLHB5 antibody

Antibodies; Transcription Factors

References

Stevens, J.D., et al. Differentiation 76(9):1006-1022(2008). McLellan, A.S., et al. Mech. Dev. 119 SUPPL 1, S285-S291 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 22748877
• NCBI GeneID: 27319
• NCBI Accession #: NP_689627.1
• NCBI GenBank Nucleotide #: NM_152414.4
• UniProt Accession #: Q8NFJ8
• Molecular Weight: 37kDa
• NCBI Official Full Name: class E basic helix-loop-helix protein 22
• NCBI Official Synonym Full Names: basic helix-loop-helix family member e22
• NCBI Official Symbol: BHLHE22
• NCBI Official Synonym Symbols: Beta3; BHLHB5; Beta3a; CAGL85; TNRC20
• NCBI Protein Information: class E basic helix-loop-helix protein 22
• UniProt Protein Name: Class E basic helix-loop-helix protein 22
• UniProt Gene Name: BHLHE22
• UniProt Synonym Gene Names: BHLHB5; TNRC20; bHLHe22; bHLHb5

NCBI Description

This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors that regulate cell fate determination, proliferation, and differentiation. A similar protein in mouse is required for the development of the dorsal cochlear nuclei, and is thought to play a role in in the differentiation of neurons involved in sensory input. The mouse protein also functions in retinogenesis. [provided by RefSeq, Oct 2016]

Uniprot Description

Inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. Despite the presence of an intact basic domain, does not bind to DNA (). In the brain, may function as an area-specific transcription factor that regulates the postmitotic acquisition of area identities and elucidate the genetic hierarchy between progenitors and postmitotic neurons driving neocortical arealization. May be required for the survival of a specific population of inhibitory neurons in the superficial laminae of the spinal chord dorsal horn that may regulate pruritis. Seems to play a crucial role in the retinogenesis, in the specification of amacrine and bipolar subtypes. Forms with PRDM8 a transcriptional repressor complex controlling genes involved in neural development and neuronal differentiation.