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SDS-Page (Gel: 12%SDS-PAGE Lysate: 40 ug, Lane 1-5: Human muscle tissue£¬Human spleen tissue£¬Mouse Pancreas tissue£¬Human pancreas tissue and Mouse liver tissue lysates, Primary antibody:BHLHA15 antibody at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Rabbit anti-Human, Mouse BHLHA15 Polyclonal Antibody | anti-BHLHA15 antibody

BHLHA15 Antibody

Gene Names
BHLHA15; MIST1; BHLHB8
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Antigen affinity purification
Synonyms
BHLHA15; Polyclonal Antibody; BHLHA15 Antibody; MIST1; BHLHB8; anti-BHLHA15 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total BHLHA15 protein.
Purity/Purification
Antigen affinity purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
0.7mg/ml (varies by lot)
Sequence Length
189
Applicable Applications for anti-BHLHA15 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
Western Blot: 1:200-1000
Immunohistochemistry: 1: 20-100
Immunogen
Synthetic peptide of human BHLHA15
Immunogen Type
Peptide
Preparation and Storage
Store at -20 degree C

SDS-Page

(Gel: 12%SDS-PAGE Lysate: 40 ug, Lane 1-5: Human muscle tissue£¬Human spleen tissue£¬Mouse Pancreas tissue£¬Human pancreas tissue and Mouse liver tissue lysates, Primary antibody:BHLHA15 antibody at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

SDS-Page (Gel: 12%SDS-PAGE Lysate: 40 ug, Lane 1-5: Human muscle tissue£¬Human spleen tissue£¬Mouse Pancreas tissue£¬Human pancreas tissue and Mouse liver tissue lysates, Primary antibody:BHLHA15 antibody at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using BHLHA15 Antibody at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200))

Immunohistochemistry (IHC) (The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using BHLHA15 Antibody at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200))
Related Product Information for anti-BHLHA15 antibody
MIST1 (muscle, intestine and stomach expression 1), also known as BHLHB8 (basic helix-loop-helix domain containing, class B, 8), is a 189 amino acid nuclear protein expressed in liver, brain, skeletal muscle and spleen. MIST1 contains a basic helix-loop-helix (bHLH) domain and belongs to the bHLH family of transcription factors. Members of this family bind to the E-box motifs present in the promoter or enhancer regions of a variety of developmentally regulated genes and function as either transcriptional activators or transcriptional repressors. MIST1 is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins (E12 and E47) and is believed to play a role regulating the transcriptional activity of MyoD, a protein involved in the regulation of muscle cell development. More specifically, MIST1 functions as a repressor of MyoD activity, ensuring that myoblast populations do not differentiate. In addition, MIST1 is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development.
Product Categories/Family for anti-BHLHA15 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,818 Da
NCBI Official Full Name
class A basic helix-loop-helix protein 15
NCBI Official Synonym Full Names
basic helix-loop-helix family member a15
NCBI Official Symbol
BHLHA15
NCBI Official Synonym Symbols
MIST1; BHLHB8
NCBI Protein Information
class A basic helix-loop-helix protein 15
UniProt Protein Name
Class A basic helix-loop-helix protein 15
UniProt Gene Name
BHLHA15
UniProt Synonym Gene Names
BHLHB8; MIST1; bHLHa15; bHLHb8; MIST-1
UniProt Entry Name
BHA15_HUMAN

Uniprot Description

MIST1: Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E- box occupancy by homodimers. May also negatively regulate bHLH- mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E- box consensus sequence 5'-CANNTG-3'.

Chromosomal Location of Human Ortholog: 7q21.3

Biological Process: cellular response to glucose starvation; unfolded protein response

Research Articles on BHLHA15

Similar Products

Product Notes

The BHLHA15 bhlha15 (Catalog #AAA9417411) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BHLHA15 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BHLHA15 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Western Blot: 1:200-1000 Immunohistochemistry: 1: 20-100. Researchers should empirically determine the suitability of the BHLHA15 bhlha15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BHLHA15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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