Western Blot (WB)
(Western blot analysis of BHLHA15 in 3T3 cell lysate with BHLHA15 antibody at (A) 1 and (B) 2 μg/mL.)
Rabbit BHLHA15 Polyclonal Antibody | anti-BHLHA15 antibody
BHLHA15 Antibody
Gene Names
BHLHA15; MIST1; BHLHB8
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity chromatography purified via peptide column.
Synonyms
BHLHA15; Polyclonal Antibody; BHLHA15 Antibody; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E; basic helix-loop-helix family member a15; anti-BHLHA15 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
189
Applicable Applications for anti-BHLHA15 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Western blot at 1 - 2 mug/mL.
Conjugate
Unconjugated
Immunogen
BHLHA15 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human BHLHA15.
Buffer
BHLHA15 Antibody is supplied in PBS containing 0.02% sodium azide.
Species
Human
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles.
Antibodies should not be exposed to prolonged high temperatures.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles.
Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of BHLHA15 in 3T3 cell lysate with BHLHA15 antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of BHLHA15 in 3T3 cell lysate with BHLHA15 antibody at (A) 1 and (B) 2 μg/mL.)
Related Product Information for anti-BHLHA15 antibody
BHLHA15 Antibody: BHLHA15 (basic helix-loop-helix protein 15), also known as MIST1 (muscle intestine and stomach expression 1) belongs to the bHLH family of transcription factors and plays a role in regulating the transcriptional activity of MYOD1 in muscle cell development as well as serving as a key regulator of acinar cell function (1,2). BHLHA15 contains a basic helix-loop-helix (bHLH) domain and is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins. It may also negatively regulate bHLH-mediated transcription through a N-terminal repressor domain (3). It is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development (4).
Product Categories/Family for anti-BHLHA15 antibody
References
1. Lemercier C, To RQ, Swanson BJ et al. MIST1: a novel basic helix-loop-helix transcription factor exhibits a developmentally regulated expression pattern. Dev. Biol. 1997; 182:101-13. 2. Pin CL, Rukstalis JM, Johnson C, et al. The bHLH transcription factor MIST1 is required to maintain exocrine pancreas cell organization and acinar cell identity. J. Cell Biol. 2001; 155:519-30. 3. Lemercier C, To RQ, Carrasco RA et al. The basic helix-loop-helix transcription factor Mist1 functions as a transcriptional repressor of myoD. EMBO J. 1998; 17:1412-22. 4. Zhao Y, Johansson C, Tran T, et al. Identification of a basic helix-loop-helix transcription factor expressed in mammary gland alveolar cells and required for maintenance of the differentiated state. Mol. Endocrinol. 2006; 20:2187-98.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 21 kDa
Observed: 22 kDa
Observed: 22 kDa
NCBI Official Full Name
class A basic helix-loop-helix protein 15
NCBI Official Synonym Full Names
basic helix-loop-helix family member a15
NCBI Official Symbol
BHLHA15
NCBI Official Synonym Symbols
MIST1; BHLHB8
NCBI Protein Information
class A basic helix-loop-helix protein 15
UniProt Protein Name
Class A basic helix-loop-helix protein 15
Protein Family
UniProt Gene Name
BHLHA15
UniProt Synonym Gene Names
BHLHB8; MIST1; bHLHa15; bHLHb8; MIST-1
Uniprot Description
MIST1: Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E- box occupancy by homodimers. May also negatively regulate bHLH- mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E- box consensus sequence 5'-CANNTG-3'.
Chromosomal Location of Human Ortholog: 7q21.3
Biological Process: cellular response to glucose starvation; unfolded protein response
Research Articles on BHLHA15
Similar Products
Product Notes
The BHLHA15 bhlha15 (Catalog #AAA154452) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BHLHA15 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BHLHA15 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Western blot at 1 - 2 mug/mL. Researchers should empirically determine the suitability of the BHLHA15 bhlha15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BHLHA15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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