Western Blot (WB)
(Western blot analysis of Bex3 expression in MCF7 (A), mouse liver (B) whole cell lysates.)
Rabbit anti-Human, Mouse Bex3 Polyclonal Antibody | anti-BEX3 antibody
Anti-Bex3 Antibody
Gene Names
NGFRAP1; Bex; BEX3; NADE; HGR74; DXS6984E
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Purified by Immunogen Affinity Chromatography
Synonyms
Bex3; Polyclonal Antibody; Anti-Bex3 Antibody; DXS6984E; NADE; Protein BEX3; Brain-expressed X-linked protein 3; Nerve growth factor receptor-associated protein 1; Ovarian granulosa cell 13.0 kDa protein HGR74; p75NTR-associated cell death executor; anti-BEX3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Bex3 protein.
Purity/Purification
Purified by Immunogen Affinity Chromatography
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Applicable Applications for anti-BEX3 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1:500-1:2000
IF/ICC: 1:50-1:200
IF/ICC: 1:50-1:200
Immunogen
Recombinant fusion protein of human Bex3. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of Bex3 expression in MCF7 (A), mouse liver (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Bex3 expression in MCF7 (A), mouse liver (B) whole cell lysates.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of Bex3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of Bex3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Related Product Information for anti-BEX3 antibody
Rabbit polyclonal antibody to Bex3
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
111
NCBI Official Full Name
Protein BEX3
NCBI Official Synonym Full Names
nerve growth factor receptor (TNFRSF16) associated protein 1
NCBI Official Symbol
NGFRAP1
NCBI Official Synonym Symbols
Bex; BEX3; NADE; HGR74; DXS6984E
NCBI Protein Information
protein BEX3; brain-expressed X-linked protein 3; p75NTR-associated cell death executor; ovarian granulosa cell 13.0 kDa protein HGR74
UniProt Protein Name
Protein BEX3
Protein Family
UniProt Gene Name
NGFRAP1
UniProt Synonym Gene Names
BEX3; DXS6984E; NADE
UniProt Entry Name
BEX3_HUMAN
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Product Notes
The BEX3 ngfrap1 (Catalog #AAA8307432) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Bex3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Bex3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:2000 IF/ICC: 1:50-1:200. Researchers should empirically determine the suitability of the BEX3 ngfrap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bex3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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