Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ARRB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Rabbit beta-Arrestin 1 Polyclonal Antibody | anti-ARRB1 antibody
beta-Arrestin 1 Rabbit pab Antibody
Gene Names
ARRB1; ARB1; ARR1
Reactivity
Human, mouse, rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
beta-Arrestin 1; Polyclonal Antibody; beta-Arrestin 1 Rabbit pab Antibody; ARRB1; beta-Arrestin 1 ; b-Arrestin 1 ; ARB1; ARR1; anti-ARRB1 antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
Sequence Length
418
Applicable Applications for anti-ARRB1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Western Blot (WB): 1/500-1000
Immunohistochemistry (IHC): 1/50-100
Immunofluorescence (IF): 1/20-50
Immunohistochemistry (IHC): 1/50-100
Immunofluorescence (IF): 1/20-50
Immunogen
A Fusion protein of human beta-Arrestin 1
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ARRB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ARRB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat kidney using ARRB1 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat kidney using ARRB1 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human esophagus using ARRB1 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human esophagus using ARRB1 antibody at dilution of 1:100 (40x lens).)
Related Product Information for anti-ARRB1 antibody
Rabbit polyclonal to Human beta-Arrestin 1
Arrestin proteins function as negative regulators of G protein coupled receptor (GPCR) signaling. Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylated GPCR and the eventual internalization of the receptor and desensitization of GPCR signaling (1). Four distinct mammalian arrestin proteins are known. Arrestin 1 (also known as S-arrestin) and arrestin 4 (or X-arrestin) are localized to retinal rods and cones, respectively. Arrestin 2 (also known as beta-arrestin 1) and arrestin 3 (or beta-arrestin 2) are ubiquitously expressed and bind to most GPCRs (2). beta-arrestin proteins function as adapters and scaffold proteins and play important roles in other processes, such as recruiting c-Src family proteins to GPCRs in ERK activation pathways (3,4). beta-arrestins are also involved in some receptor tyrosine kinase signaling pathways (5-8). Additional evidence suggests that beta-arrestin proteins translocate to the nucleus and help regulate transcription by binding transcriptional cofactors (9,10).
Arrestin proteins function as negative regulators of G protein coupled receptor (GPCR) signaling. Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylated GPCR and the eventual internalization of the receptor and desensitization of GPCR signaling (1). Four distinct mammalian arrestin proteins are known. Arrestin 1 (also known as S-arrestin) and arrestin 4 (or X-arrestin) are localized to retinal rods and cones, respectively. Arrestin 2 (also known as beta-arrestin 1) and arrestin 3 (or beta-arrestin 2) are ubiquitously expressed and bind to most GPCRs (2). beta-arrestin proteins function as adapters and scaffold proteins and play important roles in other processes, such as recruiting c-Src family proteins to GPCRs in ERK activation pathways (3,4). beta-arrestins are also involved in some receptor tyrosine kinase signaling pathways (5-8). Additional evidence suggests that beta-arrestin proteins translocate to the nucleus and help regulate transcription by binding transcriptional cofactors (9,10).
References
1. Shenoy, S.K. and Lefkowitz, R.J. (2005) Sci STKE 2005, cm10. 2. Lefkowitz, R.J. and Shenoy, S.K. (2005) Science 308, 512-7. 3. Luttrell, L.M. et al. (1999) Science 283, 655-61. 4. Luttrell, L.M. et al. (1999) Curr Opin Cell Biol 11, 177-83. 5. Luttrell, L.M. and Lefkowitz, R.J. (2002) J Cell Sci 115, 455-65. 6. Waters, C. et al. (2004) Semin Cell Dev Biol 15, 309-23. 7. Lefkowitz, R.J. and Whalen, E.J. (2004) Curr Opin Cell Biol 16, 162-8. 8. Waters, C.M. et al. (2005) Cell Signal 17, 263-77. 9. Kang, J. et al. (2005) Cell 123, 833-47. 10. Ma, L. and Pei, G. (2007) J Cell Sci 120, 213-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
418
NCBI Official Full Name
beta-arrestin-1 isoform A
NCBI Official Synonym Full Names
arrestin, beta 1
NCBI Official Symbol
ARRB1
NCBI Official Synonym Symbols
ARB1; ARR1
NCBI Protein Information
beta-arrestin-1; arrestin 2
UniProt Protein Name
Beta-arrestin-1
Protein Family
UniProt Gene Name
ARRB1
UniProt Synonym Gene Names
ARR1
UniProt Entry Name
ARRB1_HUMAN
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Product Notes
The ARRB1 arrb1 (Catalog #AAA125268) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The beta-Arrestin 1 Rabbit pab Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Arrestin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western Blot (WB): 1/500-1000 Immunohistochemistry (IHC): 1/50-100 Immunofluorescence (IF): 1/20-50. Researchers should empirically determine the suitability of the ARRB1 arrb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Arrestin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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