Rabbit anti-Human, Mouse ARRB1 Polyclonal Antibody | anti-ARRB1 antibody

ARRB1 Polyclonal Antibody

Cat. Num. AAA126876

• Gene Names: ARRB1; ARB1; ARR1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: ARRB1; Polyclonal Antibody; ARRB1 Polyclonal Antibody; ARB1; ARR1; anti-ARRB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 418

• Applicable Applications for anti-ARRB1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:20 - 1:100
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human ARRB1
• Calculated Molecular Weight: 47kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ARRB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells using ARRB1 antibody. Blue: DAPI for nuclear staining.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of 200ug extracts of K-562 cells using 3ug ARRB1 antibody. Western blot was performed from the immunoprecipitate using ARRB1 antibody at a dilition of 1:1000.)

Related Product Information for anti-ARRB1 antibody

Arrestin proteins function as negative regulators of G protein-coupled receptor (GPCR) signaling. Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylated GPCR and the eventual internalization of the receptor and desensitization of GPCR signaling (1). Four distinct mammalian arrestin proteins are known. Arrestin 1 (also known as S-arrestin) and arrestin 4 (X-arrestin) are localized to retinal rods and cones, respectively. Arrestin 2 (also known as beta-arrestin 1) and arrestin 3 (beta-arrestin 2) are ubiquitously expressed and bind to most GPCRs (2). beta-arrestins function as adaptor and scaffold proteins and play important roles in other processes, such as recruiting c-Src family proteins to GPCRs in Erk activation pathways (3,4). beta-arrestins are also involved in some receptor tyrosine kinase signaling pathways (5-8). Additional evidence suggests that beta-arrestins translocate to the nucleus and help regulate transcription by binding transcriptional cofactors (9,10).

Product Categories/Family for anti-ARRB1 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 20141238
• NCBI GeneID: 408
• UniProt Accession #: P49407; O75625; O75630; Q2PP20; Q9BTK8; B6V9G8
• Molecular Weight: 418
• NCBI Official Full Name: Beta-arrestin-1
• NCBI Official Synonym Full Names: arrestin, beta 1
• NCBI Official Symbol: ARRB1
• NCBI Official Synonym Symbols: ARB1; ARR1
• NCBI Protein Information: beta-arrestin-1; arrestin 2
• UniProt Protein Name: Beta-arrestin-1
• Protein Family: Beta-arrestin
• UniProt Gene Name: ARRB1
• UniProt Synonym Gene Names: ARR1
• UniProt Entry Name: ARRB1_HUMAN

NCBI Description

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described. [provided by RefSeq, Jan 2011]

Uniprot Description

ARRB1: regulates G-protein coupled receptors (GPCR) signaling by mediating both receptor desensitization and resensitization processes. Binds to GRK-phosphorylated receptor and sterically preclude its coupling to the cognate G- protein; the binding appears to require receptor determinants exposed only in the active receptor conformation. Targets many receptors for internalization by acting as endocytic adapters (CLASPs, clathrin-associated sorting proteins). Internalized arrestin-receptor complexes traffic to intracellular endosomes, where they remain uncoupled from G-proteins. Two different modes of arrestin-mediated internalization occur. Beta-arrestins function as multivalent adapter proteins that can switch the GPCR from a G-protein signaling mode that transmits short-lived signals from the plasma membrane via small molecule second messengers and ion channels to a beta-arrestin signaling mode that transmits a distinct set of signals that are initiated as the receptor internalizes and transits the intracellular compartment. Also involved in regulation of receptors other than GPCRs. Involved in Toll-like receptor and IL-1 receptor signaling through the interaction with TRAF6 which prevents TRAF6 autoubiquitination and oligomerization required for activation of NF-kappa-B and JUN. Binds phosphoinositides. Binds inositolhexakisphosphate (InsP6). Involved in IL8-mediated granule release in neutrophils. Interacts with phosphorylated ADRB2 and CHRM2. Interacts with SRC (via the SH3 domain and the protein kinase domain); the interaction is independent of the phosphorylation state of SRC C-terminus. Interacts with RAF1, CHUK, IKBKB and Nik. Interacts with DVL1 and DVL2; the interaction is enhanced by DVL phosphorylation. Interacts with IGF1R. Belongs to the arrestin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cytoplasmic vesicle membrane; lysosomal membrane; coated pit; pseudopodium; cytosol; Golgi membrane; nucleoplasm; cytoplasm; plasma membrane; heterotrimeric G-protein complex; cytoplasmic vesicle; nucleus; chromatin

Molecular Function: enzyme inhibitor activity; angiotensin receptor binding; insulin-like growth factor receptor binding; protein binding; histone acetyltransferase activity; mitogen-activated protein kinase kinase binding; ubiquitin protein ligase binding; caspase inhibitor activity; transcription factor binding; GTPase activator activity

Biological Process: transcription from RNA polymerase II promoter; platelet activation; proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; positive regulation of protein binding; positive regulation of receptor internalization; protein ubiquitination; positive regulation of Rho protein signal transduction; negative regulation of caspase activity; positive regulation of peptidyl-serine phosphorylation; protein transport; G-protein coupled receptor internalization; inhibition of NF-kappaB transcription factor; stress fiber formation; negative regulation of interleukin-6 production; phototransduction; negative regulation of interleukin-8 production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; negative regulation of protein ubiquitination; post-Golgi vesicle-mediated transport; positive regulation of protein amino acid phosphorylation; blood coagulation; positive regulation of GTPase activity