Immunofluorescence
(Immunofluorescent staining of beta 2 Microglobulin antibodyImmunofluorescence microscopy using beta 2 Microglobulin antibody, showing detection of beta 2 microglobulin in HEK 293 cells. Cells were cultured in a six well plate and fixed with 2% paraformaldehyde for 15 minutes, then incubated with primary antibody diluted 1:200 overnight at 4 deg C. Following washing with DPBS, Donkey anti-Rabbit (Alexa Fluor 488) secondary antibody (1:1000) was used. An inverted fluorescence microscope equipped with differential Interference Contrast (DIC) mode was used to capture the image. beta 2 microglobulin, mainly on the cell surface but to some extent cytoplasmic, is stained green. )
Rabbit beta 2 Microglobulin Polyclonal Antibody | anti-B2M antibody
beta 2 Microglobulin antibody
IHC: 1:500-1:2,000
WB: 1:1,000-1:5,000
Immunofluorescence
(Immunofluorescent staining of beta 2 Microglobulin antibodyImmunofluorescence microscopy using beta 2 Microglobulin antibody, showing detection of beta 2 microglobulin in HEK 293 cells. Cells were cultured in a six well plate and fixed with 2% paraformaldehyde for 15 minutes, then incubated with primary antibody diluted 1:200 overnight at 4 deg C. Following washing with DPBS, Donkey anti-Rabbit (Alexa Fluor 488) secondary antibody (1:1000) was used. An inverted fluorescence microscope equipped with differential Interference Contrast (DIC) mode was used to capture the image. beta 2 microglobulin, mainly on the cell surface but to some extent cytoplasmic, is stained green. )
Immunofluorescence
(Immunofluorescent staining of beta 2 Microglobulin antibodyImmunofluorescence microscopy using beta 2 Microglobulin antibody, showing detection of beta 2 microglobulin in HEK 293 cells. Cells were cultured in a six well plate and fixed with 2% paraformaldehyde for 15 minutes, then incubated with primary antibody diluted 1:200 overnight at 4 deg C. Following washing with DPBS, Donkey anti-Rabbit (Alexa Fluor 488) secondary antibody (1:1000) was used. An inverted fluorescence microscope equipped with differential Interference Contrast (DIC) mode was used to capture the image. beta 2 microglobulin, mainly on the cell surface but to some extent cytoplasmic, is stained green. )
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]
Uniprot Description
B2M: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Heterodimer of an alpha chain and a beta chain. Beta-2- microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. Belongs to the beta-2-microglobulin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 15q21.1
Cellular Component: Golgi apparatus; extracellular space; focal adhesion; endoplasmic reticulum lumen; extracellular region; Golgi membrane; phagocytic vesicle membrane; membrane; early endosome membrane; cytoplasm; plasma membrane; MHC class I protein complex; external side of plasma membrane
Molecular Function: identical protein binding; protein binding
Biological Process: response to drug; regulation of immune response; viral reproduction; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; T cell differentiation in the thymus; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; regulation of defense response to virus by virus; retinal homeostasis; iron ion homeostasis; antigen processing and presentation of peptide antigen via MHC class I; response to cadmium ion; response to molecule of bacterial origin; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein refolding; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent
Disease: Hypoproteinemia, Hypercatabolic
Research Articles on B2M
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Product Notes
The B2M b2m (Catalog #AAA534872) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The beta 2 Microglobulin antibody reacts with Human beta-2-macroglobulin and may cross-react with other species as described in the data sheet. AAA Biotech's beta 2 Microglobulin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). ELISA: 1:2,000-1:10,000 IHC: 1:500-1:2,000 WB: 1:1,000-1:5,000. Researchers should empirically determine the suitability of the B2M b2m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta 2 Microglobulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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