Rabbit beta-1, 4-Gal-T3 Polyclonal Antibody | anti-F304 antibody

beta-1, 4-Gal-T3 (F304) Polyclonal Antibody

Cat. Num. AAA3003402

• Gene Names: B4GALT3; beta4Gal-T3
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: beta-1; 4-Gal-T3; Polyclonal Antibody; 4-Gal-T3 (F304) Polyclonal Antibody; Beta-1; 4-galactosyltransferase 3; 4-GalTase 3; Beta4Gal-T3; b4Gal-T3; UDP-Gal: beta-GlcNAc beta-1; UDP-galactose: beta-N-acetylglucosamine; N-acetyllactosamine synthase; Nal synthase; Beta-N-acetylglucosaminylglycopeptide beta-1; 4-galactosyltransferase; Beta-N-acetylglucosaminyl-glycolipid beta-1; B4GALT3; anti-F304 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: beta-1, 4-Gal-T3 (F304) polyclonal antibody detects endogenous levels ofbeta-1, 4-Gal-T3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 393

• Applicable Applications for anti-F304 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 281-320 of Human beta-1, 4-Gal-T3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of beta-1,4-Gal-T3 (F304) pAb in paraffin-embedded liver cancer tissue at 1:100.)

Related Product Information for anti-F304 antibody

The beta-1, 3 galactosyltransferase(beta3GalT) gene family encodes a set of type II transmembrane glycoproteins that are catalytically diverse and use different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine) to catalyze the addition of an activated monosaccharide to a terminal lactose. The protein coding sequences for beta-1, 3-Gal-T genes comprise a single exon and are distantly related to the Drosophila Brainiac gene. The beta-1, 4-galactosyltransferase (beta4GalT) gene family encodes type II membrane-bound glycoproteins that show exclusive specificity for the donor substrate, UDP-galactose. beta-1, 4Gal-T genes transfer galactose in a beta-1, 4 linkage to similar acceptor

NCBI and Uniprot Product Information

• NCBI GI #: 315467841
• NCBI GeneID: 8703
• NCBI Accession #: NP_001186802.1
• NCBI GenBank Nucleotide #: NP_001186802.1
• UniProt Accession #: O60512
• Molecular Weight: ~ 49kDa
• NCBI Official Full Name: beta-1,4-galactosyltransferase 3
• NCBI Official Synonym Full Names: beta-1,4-galactosyltransferase 3
• NCBI Official Symbol: B4GALT3
• NCBI Official Synonym Symbols: beta4Gal-T3
• NCBI Protein Information: beta-1,4-galactosyltransferase 3
• UniProt Protein Name: Beta-1,4-galactosyltransferase 3
• UniProt Gene Name: B4GALT3
• UniProt Synonym Gene Names: Beta-1,4-GalTase 3; Beta4Gal-T3; b4Gal-T3
• UniProt Entry Name: B4GT3_HUMAN

NCBI Description

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene encodes an enzyme that may be mainly involved in the synthesis of the first N-acetyllactosamine unit of poly-N-acetyllactosamine chains. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

B4GALT3: Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Belongs to the glycosyltransferase 7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; EC 2.4.1.90; Glycan Metabolism - keratan sulfate biosynthesis; EC 2.4.1.38; Membrane protein, integral; Transferase; Glycan Metabolism - N-glycan biosynthesis

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: Golgi membrane; Golgi apparatus; cytoplasm; integral to membrane

Molecular Function: beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity; N-acetyllactosamine synthase activity; metal ion binding; galactosyltransferase activity

Biological Process: keratan sulfate metabolic process; cellular protein metabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; keratan sulfate biosynthetic process; pathogenesis; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification