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Western Blot (WB) (Western blot analysis of BEST2 Antibody (C-term) in MDA-MB435,MDA-MB231 cell line lysates (35ug/lane).BEST2 (arrow) was detected using the purified Pab.)

Rabbit anti-Human BEST2 Polyclonal Antibody | anti-BEST2 antibody

BEST2, CT (BEST2, VMD2L1, Bestrophin-2, Vitelliform macular dystrophy 2-like protein 1) (PE)

Gene Names
BEST2; VMD2L1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
BEST2; Polyclonal Antibody; CT (BEST2; VMD2L1; Bestrophin-2; Vitelliform macular dystrophy 2-like protein 1) (PE); Vitelliform macular dystrophy 2-like protein 1; anti-BEST2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-BEST2 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
BEST2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381~411 amino acids from the C-terminal region of human BEST2.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of BEST2 Antibody (C-term) in MDA-MB435,MDA-MB231 cell line lysates (35ug/lane).BEST2 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of BEST2 Antibody (C-term) in MDA-MB435,MDA-MB231 cell line lysates (35ug/lane).BEST2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma with BEST2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human breast carcinoma with BEST2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Related Product Information for anti-BEST2 antibody
BEST2 is a member of the bestrophin gene family of anion channels. Bestrophin genes share a similar gene structure with highly conserved exon-intron boundaries, but with distinct 3' ends. Bestrophins are transmembrane proteins that contain a homologous region rich in aromatic residues, including an invariant arg-phe-pro motif.
Product Categories/Family for anti-BEST2 antibody
References
Zhang, Y., et.al, Mol. Vis. 16, 200-206 (2010). Marsey, L.L. et.al, J. Physiol. (Lond.) 587 (PT 10), 2211-2224 (2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57,139 Da
NCBI Official Full Name
bestrophin-2
NCBI Official Synonym Full Names
bestrophin 2
NCBI Official Symbol
BEST2
NCBI Official Synonym Symbols
VMD2L1
NCBI Protein Information
bestrophin-2; VMD2-like gene 1; vitelliform macular dystrophy 2-like 1; vitelliform macular dystrophy 2-like protein 1
UniProt Protein Name
Bestrophin-2
Protein Family
UniProt Gene Name
BEST2
UniProt Synonym Gene Names
VMD2L1
UniProt Entry Name
BEST2_HUMAN

NCBI Description

This gene is a member of the bestrophin gene family of anion channels. Bestrophin genes share a similar gene structure with highly conserved exon-intron boundaries, but with distinct 3' ends. Bestrophins are transmembrane proteins that contain a homologous region rich in aromatic residues, including an invariant arg-phe-pro motif. Mutation in one of the family members (bestrophin 1) is associated with vitelliform macular dystrophy. The bestrophin 2 gene is mainly expressed in the retinal pigment epithelium and colon. [provided by RefSeq, Jul 2008]

Uniprot Description

BEST2: Forms calcium-sensitive chloride channels. Permeable to bicarbonate. Belongs to the bestrophin family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: plasma membrane; cilium

Molecular Function: chloride channel activity

Biological Process: membrane depolarization; sensory perception of smell

Research Articles on BEST2

Similar Products

Product Notes

The BEST2 best2 (Catalog #AAA6278262) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BEST2, CT (BEST2, VMD2L1, Bestrophin-2, Vitelliform macular dystrophy 2-like protein 1) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BEST2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-100 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BEST2 best2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BEST2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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