Rabbit anti-Human, Mouse Bcr Polyclonal Antibody | anti-Bcr antibody

Phospho-Bcr (Tyr177) Antibody

Cat. Num. AAA9600926

• Gene Names: BCR; ALL; CML; PHL; BCR1; D22S11; D22S662
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
• Synonyms: Bcr; Polyclonal Antibody; Phospho-Bcr (Tyr177) Antibody; ALL; bcr; BCR/ABL FUSION GENE; INCLUDED; BCR/FGFR1 chimera protein; BCR/FGFR1 FUSION GENE; BCR/PDGFRA FUSION GENE; BCR_HUMAN; BCR1; Breakpoint cluster region; Breakpoint cluster region protein; CML; D22S11; D22S662; FGFR1/BCR chimera protein; PHL; Renal carcinoma antigen NY-REN-26; anti-Bcr antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-Bcr (Tyr177) antibody detects endogenous levels of Bcr only when phosphorylated at Tyrosine 177
• Purity/Purification: From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1271

• Applicable Applications for anti-Bcr antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide derived from human Bcr around the phosphorylation site of Tyrosine 177
• Subcellular Location: Plasma Membrane
• Predicted Cross Reactivity: Zebrafish, Chicken
• Similarity: Zebrafish (89%), Chicken (89%)
• Conjugation: Unconjugated
• Epitope: Phospho Tyr177
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of Bcr phosphorylation expression in K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

Immunofluorescene (IF)

(MBS9600926 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Western Blot (WB)

(Western blot analysis of Phospho-Bcr (Tyr177) expression in various lysates)

Related Product Information for anti-Bcr antibody

Description: Bcr is a serine/threonine and GTPase-activating protein (GAP). breakpoint cluster region protein. GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them.
Function: GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
Subunit Structure: Homotetramer. Interacts with PDZK1. May interact with CCPG1 (By similarity). Interacts with FES/FPS, ABL1, PIK3R1 and GRB2. Interacts with HCK. Interacts with SH2D5 (PubMed:25331951).
Post-translational Modifications: Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
Similarity: The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.The DH domain is involved in interaction with CCPG1.

NCBI and Uniprot Product Information

• NCBI GI #: 82546843
• NCBI GeneID: 613
• NCBI Accession #: NP_004318.3
• NCBI GenBank Nucleotide #: NM_004327.3
• UniProt Accession #: P11274; P78501; Q12842; Q4LE80; Q6NZI3
• Molecular Weight: Observed: 160 kDa; Predicted: 143 kDa
• NCBI Official Full Name: breakpoint cluster region protein isoform 1
• NCBI Official Synonym Full Names: BCR, RhoGEF and GTPase activating protein
• NCBI Official Symbol: BCR
• NCBI Official Synonym Symbols: ALL; CML; PHL; BCR1; D22S11; D22S662
• NCBI Protein Information: breakpoint cluster region protein
• UniProt Protein Name: Breakpoint cluster region protein
• Protein Family: Bicyclomycin resistance protein
• UniProt Gene Name: BCR
• UniProt Synonym Gene Names: BCR1; D22S11

NCBI Description

A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.