Rabbit Bcl6 Polyclonal Antibody | anti-Bcl6 antibody

Bcl6 Antibody

Cat. Num. AAA9613693

• Gene Names: BCL6; BCL5; LAZ3; BCL6A; ZNF51; ZBTB27
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: Bcl6; Polyclonal Antibody; Bcl6 Antibody; B cell CLL/lymphoma 6; B cell lymphoma 6 protein; B-cell lymphoma 5 protein; B-cell lymphoma 6 protein; BCL 5; Bcl 6; BCL-5; BCL-6; BCL5; BCL6; BCL6_HUMAN; BCL6A; cys his2 zinc finger transcription factor; LAZ 3; LAZ 3 protein; LAZ3; Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3); Lymphoma associated zinc finger gene on chromosome 3; Protein LAZ-3; ZBTB 27; ZBTB27; Zinc finger and BTB domain containing protein 27; Zinc finger and BTB domain-containing protein 27 (ZBTB27); Zinc finger and BTB domain-containing protein 27; Zinc Finger Protein 51 (ZNF51); Zinc finger protein 51; zinc finger transcription factor BCL6S; ZNF 51; ZNF51; anti-Bcl6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Bcl6 Antibody detects endogenous levels of total Bcl6.; Tissue Specificity: Expressed in germinal center T- and B-cells and in primary immature dendritic cells.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-Bcl6 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Bcl6.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated by MAPK1 in response to antigen receptor activation at Ser-333 and Ser-343. Phosphorylated by ATM in response to genotoxic stress. Phosphorylation induces its degradation by ubiquitin/proteasome pathway.Polyubiquitinated. Polyubiquitinated by SCF (FBXO11), leading to its degradation by the proteasome. Ubiquitinated by the SCF (FBXL17) complex, leading to its degradation by the proteaseome: ubiquitination by the SCF (FBXL17) complex takes place when aberrant BTB domain dimers are formed. Acetylated at Lys-379 by EP300 which inhibits the interaction with NuRD complex and the transcriptional repressor function. Deacetylated by HDAC- and SIR2-dependent pathways.
• Subunit Structure: Homodimer. Interacts (via BTB domain) with the corepressors BCOR, NCOR1 and SMRT/NCOR2; the interactions are direct. Forms preferably ternary complexes with BCOR and SMRT/NCOR2 on target gene promoters but, on enhancer elements, interacts with SMRT/NCOR2 and HDAC3 to repress proximal gene expression. Interacts with histone deacetylases HDAC2, HDAC5 and HDAC9 (via the catalytic domain). Interacts with ZBTB7 and BCL6B. Interacts with SCF (FBXO11) complex; the interaction is independent of phosphorylation and promotes ubiquitination. Interacts (when phosphorylated) with PIN1; the interaction is required for BCL6 degradation upon genotoxic stress. Interacts with ZBTB17; inhibits ZBTB17 transcriptional activity. Interacts with CTBP1, autoinhibits its transcriptional expression. Interacts with NOTCH1 NCID and SIRT1; leads to a epigenetic repression of selective NOTCH1-target genes. Interacts (nor via BTB domain neither acetylated) with the NuRD complex components CHD4, HDAC1, MBD3 and MTA3; the interaction with MTA3 inhibits BCL6 acetylation and is required for BCL6 transpriptional repression.
• Similarity: The BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2 (PubMed:18212045). Interaction with corepressors through the BTB domain is needed to facilitate the rapid proliferation and survival of GC B-cells but is not involved in the T (FH) formation and BCL6-mediated suppression of T (H)2 and T (H)17 differentiationrequired for GC formation (By similarity).
• Subcellular Location: Nucleus.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Related Product Information for anti-Bcl6 antibody

Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4 (+) T-cells (T (FH) cells), promotes the expression of T (FH)-related genes but inhibits the differentiation of T (H)1, T (H)2 and T (H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.

NCBI and Uniprot Product Information

• NCBI GI #: 195927002
• NCBI GeneID: 604
• NCBI Accession #: NP_001124317.1
• NCBI GenBank Nucleotide #: NM_001130845.1
• UniProt Accession #: P41182; A7E241; B8PSA7; D3DNV5
• Molecular Weight: 78,846 Da
• NCBI Official Full Name: B-cell lymphoma 6 protein isoform 1
• NCBI Official Synonym Full Names: B-cell CLL/lymphoma 6
• NCBI Official Symbol: BCL6
• NCBI Official Synonym Symbols: BCL5; LAZ3; BCL6A; ZNF51; ZBTB27
• NCBI Protein Information: B-cell lymphoma 6 protein; BCL-5; BCL-6; protein LAZ-3; zinc finger protein 51; B-cell lymphoma 5 protein; B-cell lymphoma 6 protein transcript; zinc finger transcription factor BCL6S; cys-his2 zinc finger transcription factor; zinc finger and BTB domain-
• UniProt Protein Name: B-cell lymphoma 6 protein
• Protein Family: B-cell lymphoma 6 protein
• UniProt Gene Name: BCL6
• UniProt Synonym Gene Names: BCL5; LAZ3; ZBTB27; ZNF51; BCL-6; BCL-5
• UniProt Entry Name: BCL6_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

Bcl-6: a zinc finger transcription factor and contains an N-terminal BTB/POZ domain. A sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. Mediates transcriptional repression and interacts with components of histone deacetylase co-repressor complexes including N-CoR and SMRT. The gene is frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL.

Protein type: C2H2-type zinc finger protein; Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: replication fork; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; chromatin DNA binding; metal ion binding; chromatin binding; transcription factor activity

Biological Process: erythrocyte development; positive regulation of apoptosis; regulation of memory T cell differentiation; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; Rho protein signal transduction; negative regulation of cell proliferation; negative regulation of isotype switching to IgE isotypes; positive regulation of histone deacetylation; positive regulation of B cell proliferation; germinal center formation; negative regulation of mast cell cytokine production; inflammatory response; negative regulation of T-helper 2 cell differentiation; regulation of immune response; T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of cell motility; negative regulation of cell-matrix adhesion; regulation of germinal center formation; negative regulation of Rho protein signal transduction; protein import into nucleus, translocation; B cell differentiation; regulation of inflammatory response; negative regulation of Notch signaling pathway; spermatogenesis; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; positive regulation of neuron differentiation; actin cytoskeleton organization and biogenesis; response to DNA damage stimulus; negative regulation of B cell apoptosis