Rabbit anti-Human, Mouse BCL3 Polyclonal Antibody | anti-BCL3 antibody

BCL3, ID (BCL3, BCL4, D19S37, B Cell lymphoma 3 protein, Proto-oncogene BCL3) (Biotin)

Cat. Num. AAA6277990

• Gene Names: BCL3; BCL4; D19S37
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BCL3; Polyclonal Antibody; ID (BCL3; BCL4; D19S37; B Cell lymphoma 3 protein; Proto-oncogene BCL3) (Biotin); B-cell lymphoma 3 protein; Proto-oncogene BCL3; anti-BCL3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BCL3. Species crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-BCL3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a portion within aa249-277 from the center region of human BCL3, conjugated to KLH (P20749).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of BCL3 Antibody (Center) in mouse liver tissue lysates (35ug/lane). BCL3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with MBS643685 (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of BCL3 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(BCL3 Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-BCL3 antibody

BCL3 is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers.

Product Categories/Family for anti-BCL3 antibody

Antibodies; Cancer Markers

References

1. Kabuta, T. et.al., Biochem. Biophys. Res. Commun. 394 (3), 697-702 (2010). 2. Talmud, P.J. et.al., Am. J. Hum. Genet. 85 (5), 628-642 (2009). 3. Folco, E.J. et.al., J. Biol. Chem. 284 (38), 25569-25575 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 164664508
• NCBI GeneID: 602
• NCBI Accession #: NP_005169.2
• NCBI GenBank Nucleotide #: NM_005178.4
• UniProt Accession #: P20749
• Molecular Weight: 47,584 Da
• NCBI Official Full Name: B-cell lymphoma 3 protein
• NCBI Official Synonym Full Names: B-cell CLL/lymphoma 3
• NCBI Official Symbol: BCL3
• NCBI Official Synonym Symbols: BCL4; D19S37
• NCBI Protein Information: B-cell lymphoma 3 protein; BCL-3; proto-oncogene BCL3; B-cell leukemia/lymphoma 3; B-cell lymphoma 3-encoded protein; chronic lymphatic leukemia protein
• UniProt Protein Name: B-cell lymphoma 3 protein
• Protein Family: B-cell lymphoma 3 protein
• UniProt Gene Name: BCL3
• UniProt Synonym Gene Names: BCL4; D19S37; BCL-3
• UniProt Entry Name: BCL3_HUMAN

NCBI Description

This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation

By similarity. Ref.4

Subunit structure: Component of a complex consisting of the NF-kappa-B p52-p52 homodimer and BCL3. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Interacts with N4BP2, COPS5 and PIR. Interacts with CYLD

By similarity. Ref.4 Ref.5 Ref.6 Ref.7

Subcellular location: Nucleus. Cytoplasm

By similarity. Cytoplasm › perinuclear region

By similarity. Note: Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation

By similarity.

Post-translational modification: Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation

By similarity.Activated by phosphorylation.

Involvement in disease: A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions.

Sequence similarities: Contains 7 ANK repeats.

Caution: It is uncertain whether Met-1 or Met-9 is the initiator.

Sequence caution: The sequence AAA51815.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAA51816.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH64993.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.