Rabbit BCL2L2 Polyclonal Antibody | anti-BCL2L2 antibody

BCL2L2 (BCL2-like 2 Apoptosis Facilitator, BCL-W, BCLW, KIAA0271) (Biotin)

Cat. Num. AAA6408403

• Gene Names: BCL2L2; BCLW; BCL-W; PPP1R51; BCL2-L-2
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: BCL2L2; Polyclonal Antibody; BCL2L2 (BCL2-like 2 Apoptosis Facilitator; BCL-W; BCLW; KIAA0271) (Biotin); anti-BCL2L2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes BCL2L2. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-BCL2L2 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human BCL2L2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HL-60 cell lysate using BCL2L2 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human kidney carcinoma tissue using BCL2L2 antibody.)

Product Categories/Family for anti-BCL2L2 antibody

Antibodies; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 315360668
• NCBI GeneID: 599
• NCBI Accession #: NP_001186768.1
• NCBI GenBank Nucleotide #: NM_001199839.1
• UniProt Accession #: Q92843; Q2M3U0; Q5U0H4; A8K0F4
• Molecular Weight: 20,746 Da
• NCBI Official Full Name: bcl-2-like protein 2
• NCBI Official Synonym Full Names: BCL2-like 2
• NCBI Official Symbol: BCL2L2
• NCBI Official Synonym Symbols: BCLW; BCL-W; PPP1R51; BCL2-L-2
• NCBI Protein Information: bcl-2-like protein 2; apoptosis regulator BCL-W; protein phosphatase 1, regulatory subunit 51
• UniProt Protein Name: Bcl-2-like protein 2
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: BCL2L2
• UniProt Synonym Gene Names: BCLW; KIAA0271; Bcl2-L-2
• UniProt Entry Name: B2CL2_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream PABPN1 (poly(A) binding protein, nuclear 1) gene. [provided by RefSeq, Dec 2010]

Uniprot Description

BCL2L2: Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX. Expressed in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines. Belongs to the Bcl-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 14q11.2-q12

Cellular Component: mitochondrial outer membrane; cytosol

Molecular Function: BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; Sertoli cell proliferation; spermatogenesis; negative regulation of apoptosis