Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using BCL2L13 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Rabbit anti-Human, Mouse BCL2L13 Polyclonal Antibody | anti-BCL2L13 antibody
BCL2L13 Polyclonal Antibody
Gene Names
BCL2L13; MIL1; BCL-RAMBO; Bcl2-L-13
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
BCL2L13; Polyclonal Antibody; BCL2L13 Polyclonal Antibody; MIL1; Protein Mil1; BCL-RAMBO; Bcl2-L-13; anti-BCL2L13 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
485
Applicable Applications for anti-BCL2L13 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human BCL2L13
Calculated Molecular Weight
53kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using BCL2L13 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using BCL2L13 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using BCL2L13 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using BCL2L13 antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-BCL2L13 antibody
Apoptosis is defined as a set of cascades which, when initiated, program the cell to undergo lethal changes such as membrane blebbing, mitochondrial breakdown and DNA fragmentation. The Bcl-2 family of proteins plays a central regulatory role in apoptosis. Bcl-rambo, a member of the Bcl-2 family, localizes to the mitochondria and, like other Bcl-2 family members, contains all four BH domains. Although Bcl-rambo shares structural similarity to other Bcl-2 members, it differs from them in its unique C-terminal region. Bcl-rambo has a 250 amino acid sequence containing two tandem repeats that preceeds the membrane anchor region at its C-terminus. Additionally, it is the membrane anchor C-terminal region of Bcl-rambo, not its Bcl-2 homology motifs, that is responsible for its pro-apoptotic activity. Bcl-rambo induces apoptosis when overexpressed and appears to do so by promoting mitochondrial cyto-chrome c release. It may also facilitate the activation of caspase-3.
Product Categories/Family for anti-BCL2L13 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
485
NCBI Official Full Name
Bcl-2-like protein 13
NCBI Official Synonym Full Names
BCL2-like 13 (apoptosis facilitator)
NCBI Official Symbol
BCL2L13
NCBI Official Synonym Symbols
MIL1; BCL-RAMBO; Bcl2-L-13
NCBI Protein Information
bcl-2-like protein 13
UniProt Protein Name
Bcl-2-like protein 13
Protein Family
UniProt Gene Name
BCL2L13
UniProt Synonym Gene Names
MIL1; Bcl2-L-13
UniProt Entry Name
B2L13_HUMAN
NCBI Description
This gene encodes a mitochondrially-localized protein with conserved B-cell lymphoma 2 homology motifs. Overexpression of the encoded protein results in apoptosis. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]
Uniprot Description
Bcl-2L13: May promote the activation of caspase-3 and apoptosis. Belongs to the Bcl-2 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Apoptosis
Chromosomal Location of Human Ortholog: 22q11.1
Cellular Component: mitochondrion; mitochondrial membrane; integral to membrane; nucleus
Molecular Function: caspase activator activity
Biological Process: caspase activation; apoptosis
Research Articles on BCL2L13
Similar Products
Product Notes
The BCL2L13 bcl2l13 (Catalog #AAA127575) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BCL2L13 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BCL2L13 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the BCL2L13 bcl2l13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCL2L13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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