Rabbit anti-Human BCL2A1 Polyclonal Antibody | anti-BCL2A1 antibody

BCL2A1, ID (BCL2A1, BCL2L5, BFL1, GRS, HBPA1, Bcl-2-related protein A1, Bcl-2-like protein 5, Hemopoietic-specific early response protein, Protein BFL-1, Protein GRS) (MaxLight 550)

Cat. Num. AAA6277973

• Gene Names: BCL2A1; GRS; BFL1; ACC-1; ACC-2; HBPA1; BCL2L5
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: BCL2A1; Polyclonal Antibody; ID (BCL2A1; BCL2L5; BFL1; GRS; HBPA1; Bcl-2-related protein A1; Bcl-2-like protein 5; Hemopoietic-specific early response protein; Protein BFL-1; Protein GRS) (MaxLight 550); Protein GRS; anti-BCL2A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-BCL2A1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: BCL2A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63~92 amino acids from the Center region of human BCL2A1.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of BCL2A1 Antibody (Center) in T47D cell line lysates (35ug/lane). BCL2A1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with BCL2A1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(BCL2A1 Antibody (Center) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-BCL2A1 antibody

Bcl-2 related Protein A1 is a member of the BCL-2 protein family. The proteins of this family form hetero-or homodimers and act as anti-and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and has been shown to be up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival.

Product Categories/Family for anti-BCL2A1 antibody

Antibodies; Apoptosis

References

Akatsuka, Y., et al., J. Exp. Med. 197(11):1489-1500 (2003). Edelstein, L.C., et al., Mol. Cell. Biol. 23(8):2749-2761 (2003). Werner, A.B., et al., J. Biol. Chem. 277(25):22781-22788 (2002). Akari, H., et al., J. Exp. Med. 194(9):1299-1311 (2001). Harrington, J.J., et al., Nat. Biotechnol. 19(5):440-445 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 2493280
• NCBI GeneID: 597
• UniProt Accession #: Q16548; Q6FGZ4; Q6FH19; Q86W13; Q99524
• Molecular Weight: 175
• NCBI Official Full Name: Bcl-2-related protein A1
• NCBI Official Synonym Full Names: BCL2-related protein A1
• NCBI Official Symbol: BCL2A1
• NCBI Official Synonym Symbols: GRS; BFL1; ACC-1; ACC-2; HBPA1; BCL2L5
• NCBI Protein Information: bcl-2-related protein A1; bcl2-L-5; protein BFL-1; bcl-2-like protein 5; hematopoietic BCL2-related protein A1; hemopoietic-specific early response protein
• UniProt Protein Name: Bcl-2-related protein A1
• Protein Family: Bcl-2-related protein
• UniProt Gene Name: BCL2A1
• UniProt Synonym Gene Names: BCL2L5; BFL1; GRS; HBPA1; Bcl2-L-5
• UniProt Entry Name: B2LA1_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and is up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BCL2A1: Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection. Interacts directly with BAK1, BID, BMF and BBC3. Interacts directly with BCL2L11/BIM. Interacts with BAX isoform Sigma. Interacts directly with PMAIP1. By phorbol ester and inflammatory cytokines, such as TNF or IL1B/interleukin-1 beta, but not by growth factors. Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies. Belongs to the Bcl-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 15q24.3

Cellular Component: mitochondrial outer membrane

Molecular Function: BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of apoptosis