Western Blot (WB)
(Western Blot: Sample: Human A549 Cells.)
Rabbit anti-Human Bcl2 Associated Death Promoter Polyclonal Antibody | anti-BAD antibody
Polyclonal Antibody to Bcl2 Associated Death Promoter (BAD)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MFQIPEFEPS EQEDSSSAER GLGPSPAGDG PSGSGKHHRQ APGLLWDASH QQEQPTSSSH HGGAGAVEIR SRHSSYPAGT EDDEGMGEEP SPFRGRSRSA PPNLWAAQRY GRELRRMSDE FVDSFKKGLP RPKSAGTATQ MRQSSSWTRV FQSWWDRNLG RGSSAPSQ
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Human A549 Cells.)
Western Blot (WB)
(Western Blot: Sample: Human A549 Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant BAD, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant BAD, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]
Uniprot Description
BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: cytosol; mitochondrial outer membrane; mitochondrion
Molecular Function: 14-3-3 protein binding; caspase activator activity; lipid binding; phospholipid binding; protein binding; protein heterodimerization activity; protein kinase B binding; protein kinase binding; protein phosphatase 2B binding
Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; ADP metabolic process; apoptosis; ATP metabolic process; cellular process regulating host cell cycle in response to virus; cellular response to hypoxia; cellular response to lipid; cellular response to nicotine; cerebral cortex development; cytokine-mediated signaling pathway; DNA damage response, signal transduction resulting in induction of apoptosis; glucose catabolic process; glucose homeostasis; induction of apoptosis via death domain receptors; negative regulation of cytolysis; pore complex assembly; positive regulation of apoptosis; positive regulation of apoptosis by virus; positive regulation of autophagy; positive regulation of B cell differentiation; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of epithelial cell proliferation; positive regulation of glucokinase activity; positive regulation of insulin secretion; positive regulation of intrinsic apoptotic signaling pathway; positive regulation of intrinsic apoptotic signaling pathway in response to osmotic stress; positive regulation of mitochondrial membrane potential; positive regulation of neuron death; positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway; positive regulation of proteolysis; positive regulation of release of cytochrome c from mitochondria; positive regulation of T cell differentiation; positive regulation of type B pancreatic cell development; protein insertion into mitochondrial membrane involved in apoptotic signaling pathway; regulation of mitochondrial membrane permeability; release of cytochrome c from mitochondria; response to amino acid; response to calcium ion; response to drug; response to estradiol; response to ethanol; response to glucocorticoid stimulus; response to glucose stimulus; response to hydrogen peroxide; response to oleate; response to progesterone; response to testosterone stimulus; spermatogenesis; suppression by virus of host apoptotic process; type B pancreatic cell proliferation
Research Articles on BAD
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Product Notes
The BAD bad (Catalog #AAA2026780) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Bcl2 Associated Death Promoter (BAD) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bcl2 Associated Death Promoter can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the BAD bad for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MFQIPEFEPS EQEDSSSAER GLGPSPAGDG PSGSGKHHRQ APGLLWDASH QQEQPTSSSH HGGAGAVEIR SRHSSYPAGT EDDEGMGEEP SPFRGRSRSA PPNLWAAQRY GRELRRMSDE FVDSFKKGLP RPKSAGTATQ MRQSSSWTRV FQSWWDRNLG RGSSAPSQ. It is sometimes possible for the material contained within the vial of "Bcl2 Associated Death Promoter, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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