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Western Blot (WB) (Western blot analysis of extracts of various cell lines using BCL2.)

Rabbit BCL2 Polyclonal Antibody | anti-BCL2 antibody

BCL2 (Bcl-2-related Protein A1, Bcl-2, Oncogene B-cell Leukemia 2, Apoptosis regulator Bcl-2) (MaxLight 550)

Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified by affinity chromatography.
Synonyms
BCL2; Polyclonal Antibody; BCL2 (Bcl-2-related Protein A1; Bcl-2; Oncogene B-cell Leukemia 2; Apoptosis regulator Bcl-2) (MaxLight 550); anti-BCL2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes BCL2. Species Crossreactivity: human, mouse, rat
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Applicable Applications for anti-BCL2 antibody
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
IF: 1:50-1:200
WB: 1:500-1:2000
IHC: 1:50-1:200
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human BCL2.
Conjugate
MaxLight550
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines using BCL2.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines using BCL2.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cell using BCL2. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of A549 cell using BCL2. Blue: DAPI for nuclear staining.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using BCL2 at dilution of 1:100 (x40 lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach using BCL2 at dilution of 1:100 (x40 lens).)
Product Categories/Family for anti-BCL2 antibody

NCBI and Uniprot Product Information

NCBI GeneID
596
UniProt Accession #
Molecular Weight
26
NCBI Official Synonym Full Names
BCL2 apoptosis regulator
NCBI Official Symbol
BCL2
NCBI Official Synonym Symbols
Bcl-2; PPP1R50
NCBI Protein Information
apoptosis regulator Bcl-2

NCBI Description

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Research Articles on BCL2

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Product Notes

The BCL2 (Catalog #AAA6408309) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BCL2 (Bcl-2-related Protein A1, Bcl-2, Oncogene B-cell Leukemia 2, Apoptosis regulator Bcl-2) (MaxLight 550) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BCL2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC). IF: 1:50-1:200 WB: 1:500-1:2000 IHC: 1:50-1:200 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BCL2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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