Western Blot (WB)
(Western blot analysis of BCL10 expression in MCF7 (A), A549 (B), Jurkat (C) whole cell lysates.)
Rabbit BCL10 Polyclonal Antibody | anti-BCL10 antibody
Anti-BCL10 Antibody
Western Blot (WB)
(Western blot analysis of BCL10 expression in MCF7 (A), A549 (B), Jurkat (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of BCL10 expression in MCF7 (A), A549 (B), Jurkat (C) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of BCL10 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of BCL10 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
Uniprot Description
Bcl-10: a ubiquitous pro-apoptotic protein that promotes pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Self-associates via CARD-CARD interactions; forms a tight complex with MALT1. Interacts with other CARD-proteins such as CARD9, CARD10, CARD11 and CARD14. Binds caspase-9 with its C-terminal domain. Interacts with TRAF2 and BIRC2/c-IAP2. Defects in BCL10 are involved in various types of cancer.
Protein type: Apoptosis; Tumor suppressor; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 1p22
Cellular Component: cytoplasm; cytoplasmic microtubule; cytosol; lipopolysaccharide receptor complex; lysosome; nucleus; perinuclear region of cytoplasm; plasma membrane; protein complex; T cell receptor complex
Molecular Function: enzyme binding; kinase activator activity; kinase binding; NF-kappaB binding; protease binding; protein binding; protein C-terminus binding; protein kinase B binding; protein kinase binding; protein self-association; transcription coactivator activity; transcription factor binding; ubiquitin binding; ubiquitin protein ligase binding
Biological Process: activation of NF-kappaB transcription factor; adaptive immune response; cell death; innate immune response; negative regulation of mature B cell apoptosis; neural tube closure; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-8 biosynthetic process; positive regulation of phosphorylation; positive regulation of protein ubiquitination; positive regulation of transcription, DNA-dependent; protein homooligomerization; protein oligomerization; response to food; response to molecule of bacterial origin; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; toll-like receptor signaling pathway
Disease: Follicular Lymphoma, Susceptibility To, 1; Gastric Lymphoma, Primary; Immunodeficiency 37; Lymphoma, Non-hodgkin, Familial; Mesothelioma, Malignant; Testicular Germ Cell Tumor
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Product Notes
The BCL10 bcl10 (Catalog #AAA8247268) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BCL10 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BCL10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IF/IC: 1/50 - 1/200. Researchers should empirically determine the suitability of the BCL10 bcl10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCL10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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