Testing Data
(Gel: 6%SDS-PAGE Lysates (from left to right): Hela, 231 and A431 cell Amount of lysate: 40ug per lane Primary antibody: 1/100 dilution Secondary antibody dilution: 1/8000 Exposure time: 4 minutes)
Rabbit BCAR1 Polyclonal Antibody | anti-BCAR1 antibody
BCAR1 Antibody
Gene Names
BCAR1; CAS; CAS1; CASS1; CRKAS; P130Cas
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Synonyms
BCAR1; Polyclonal Antibody; BCAR1 Antibody; CAS; CAS1; CASS1; CRKAS; P130Cas; anti-BCAR1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total BCAR1 protein.
Purity/Purification
Antigen affinity purification.
Form/Format
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Concentration
0.3 mg/ml (varies by lot)
Sequence Length
916
Applicable Applications for anti-BCAR1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western blotting: 1:200-1:1000
Immunohistochemistry: 1:25-1:100
Immunohistochemistry: 1:25-1:100
Immunogen Type
Peptide
Immunogen Description
Synthetic peptide corresponding to residues near the N terminal of human breast cancer anti-estrogen resistance 1
Target Name
BCAR1
Preparation and Storage
Store at -20 degree C
Testing Data
(Gel: 6%SDS-PAGE Lysates (from left to right): Hela, 231 and A431 cell Amount of lysate: 40ug per lane Primary antibody: 1/100 dilution Secondary antibody dilution: 1/8000 Exposure time: 4 minutes)
Testing Data
(Gel: 6%SDS-PAGE Lysates (from left to right): Hela, 231 and A431 cell Amount of lysate: 40ug per lane Primary antibody: 1/100 dilution Secondary antibody dilution: 1/8000 Exposure time: 4 minutes)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using at dilution 1/15.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using at dilution 1/15.)
Related Product Information for anti-BCAR1 antibody
BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion. The molecular cloning of p130 Cas has shown it to represent a novel SH3 containing signaling molecule with a cluster of multiple putative SH2-binding motifs for v-Crk. By immunoprecipitation analysis, p130 Cas has been shown to be highly phosphorylated at tyrosine residues subsequent to either v-Src p60 or v-Crk-mediated transformation and to form stable complexes with both of these transforming proteins. p130 Cas behaves as an extremely potent substrate for protein tyrosine kinases and has been reported to relocate from the cytoplasm to cell membrane upon tyrosine phosphorylation. One proposed model is that the SH2 domain of v-Crk functions to activate c-Src kinase, which in turn phosphorylates p130 Cas.
Product Categories/Family for anti-BCAR1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
93,291 Da
NCBI Official Full Name
breast cancer anti-estrogen resistance protein 1 isoform 1
NCBI Official Synonym Full Names
breast cancer anti-estrogen resistance 1
NCBI Official Symbol
BCAR1
NCBI Official Synonym Symbols
CAS; CAS1; CASS1; CRKAS; P130Cas
NCBI Protein Information
breast cancer anti-estrogen resistance protein 1
UniProt Protein Name
Breast cancer anti-estrogen resistance protein 1
Protein Family
UniProt Gene Name
BCAR1
UniProt Synonym Gene Names
CAS; CASS1; CRKAS
UniProt Entry Name
BCAR1_HUMAN
NCBI Description
BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009]
Uniprot Description
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
Research Articles on BCAR1
Similar Products
Product Notes
The BCAR1 bcar1 (Catalog #AAA9413475) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BCAR1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BCAR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western blotting: 1:200-1:1000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the BCAR1 bcar1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCAR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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