Rabbit anti-Human BCAM Polyclonal Antibody | anti-BCAM antibody

Anti-BCAM Antibody, Rabbit Polyclonal

Cat. Num. AAA8101927

• Gene Names: BCAM; AU; LU; CD239; MSK19
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: BCAM; Polyclonal Antibody; Anti-BCAM Antibody; Rabbit Polyclonal; BCAM Antibody; Rabbit PAb; Antigen Affinity Purified; basal cell adhesion molecule (Lutheran blood group); Anti-AU Antibody; Anti-CD239 Antibody; Anti-LU Antibody; Anti-MSK19 Antibody; anti-BCAM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human BCAM
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-BCAM antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human BCAM protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human BCAM (rh BCAM). BCAM specific IgG was purified by Human BCAM affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human BCAM in human tonsil with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to the endothelium of blood vessel walls.)

Immunohistochemistry (IHC)

(Immunochemical staining of human BCAM in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to the epithelia and endothelium.)

Immunoprecipitation (IP)

(BCAM was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-BCAM rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-BCAM rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 90 kDaObserved band size: 90 kDa)

Western Blot (WB)

(Anti-BCAM rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:67 kDaObserved band size:90 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-BCAM antibody

The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha(5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region, and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells, Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently, BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166, and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation. BCAM is upregulated following malignant transformation of some cell types in vivo and in vitro, thus being a candidate molecule involved in tumor progression. In addition, BCAM interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes.

References

Kikkawa Y, et al. (2005) Review: Lutheran/B-CAM: a laminin receptor on red blood cells and in various tissues. Connect Tissue Res. 46 (4-5): 193-9.El Nemer W, et al. (2007) Endothelial Lu/BCAM glycoproteins are novel ligands for red blood cell alpha4beta1 integrin: role in adhesion of sickle red blood cells to endothelial cells. Blood. 109 (8): 3544-51.Colin Y, et al. (2008) Red cell and endothelial Lu/BCAM beyond sickle cell disease. Transfus Clin Biol. 15 (6): 402-5.El Nemer W, et al. (2008) Role of Lu/BCAM in abnormal adhesion of sickle red blood cells to vascular endothelium. Transfus Clin Biol. 15 (1-2): 29-33.

NCBI and Uniprot Product Information

• NCBI GI #: 31543106
• NCBI GeneID: 4059
• NCBI Accession #: NP_005572.2
• NCBI GenBank Nucleotide #: NM_005581.4
• Molecular Weight: 67,405 Da
• NCBI Official Full Name: basal cell adhesion molecule isoform 1
• NCBI Official Synonym Full Names: basal cell adhesion molecule (Lutheran blood group)
• NCBI Official Symbol: BCAM
• NCBI Official Synonym Symbols: AU; LU; CD239; MSK19
• NCBI Protein Information: basal cell adhesion molecule
• Protein Family: Basal cell adhesion molecule

NCBI Description

This gene encodes Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]