Rabbit BBC3 Polyclonal Antibody | anti-BBC3 antibody

BBC3 (BCL2 Binding Component 3, p53 Upregulated Modulator of Apoptosis/Bcl 2 Binding Component 3, JFY1, PUMA, JFY-1) (FITC)

Cat. Num. AAA6408195

• Gene Names: BBC3; JFY1; PUMA; JFY-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: BBC3; Polyclonal Antibody; BBC3 (BCL2 Binding Component 3; p53 Upregulated Modulator of Apoptosis/Bcl 2 Binding Component 3; JFY1; PUMA; JFY-1) (FITC); anti-BBC3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes BBC3. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-BBC3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to human BBC3.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using BBC3 antibody.)

Product Categories/Family for anti-BBC3 antibody

Antibodies; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 56748610
• NCBI GeneID: 27113
• UniProt Accession #: Q9BXH1; O00171; Q96PG9; B9EGI3; Q96PG8
• Molecular Weight: 193
• NCBI Official Full Name: Bcl-2-binding component 3
• NCBI Official Synonym Full Names: BCL2 binding component 3
• NCBI Official Symbol: BBC3
• NCBI Official Synonym Symbols: JFY1; PUMA; JFY-1
• NCBI Protein Information: bcl-2-binding component 3; p53 up-regulated modulator of apoptosis
• UniProt Protein Name: Bcl-2-binding component 3
• Protein Family: Bcl-2-binding component
• UniProt Gene Name: BBC3
• UniProt Synonym Gene Names: PUMA
• UniProt Entry Name: BBC3_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

Uniprot Description

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; determination of adult life span; response to DNA damage stimulus; negative regulation of growth