Rabbit anti-Human BAZ1B Polyclonal Antibody | anti-BAZ1B antibody

BAZ1B, NT (BAZ1B, WBSC10, WBSCR10, WBSCR9, WSTF, Tyrosine-protein kinase BAZ1B, Bromodomain adjacent to zinc finger domain protein 1B, Williams syndrome transcription factor, Williams-Beuren syndrome chromosomal region 10 protein, Williams-Beuren syndrome

Cat. Num. AAA6277710

• Gene Names: BAZ1B; WSTF; WBSCR9; WBSCR10
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BAZ1B; Polyclonal Antibody; NT (BAZ1B; WBSC10; WBSCR10; WBSCR9; WSTF; Tyrosine-protein kinase BAZ1B; Bromodomain adjacent to zinc finger domain protein 1B; Williams syndrome transcription factor; Williams-Beuren syndrome chromosomal region 10 protein; Williams-Beuren syndrome; Williams-Beuren syndrome chromosomal region 9 protein; hWALp2; anti-BAZ1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-BAZ1B antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: BAZ1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157-186 amino acids from the N-terminal region of human BAZ1B.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-BAZ1B antibody

This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23.

Product Categories/Family for anti-BAZ1B antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Johansen, C.T., et al. Nat. Genet. 42(8):684-687(2010). Chidambaram, M., et al. Metab. Clin. Exp. (2010). In press: Oya, H., et al. J. Biol. Chem. 284(47):32472-32482(2009). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 14670392
• NCBI GeneID: 9031
• NCBI Accession #: NP_115784.1
• NCBI GenBank Nucleotide #: NM_032408.3
• UniProt Accession #: Q9UIG0; O95039; O95247; O95277; Q6P1K4; Q86UJ6; B9EGK3; D3DXE9
• Molecular Weight: 170,447 Da
• NCBI Official Full Name: tyrosine-protein kinase BAZ1B
• NCBI Official Synonym Full Names: bromodomain adjacent to zinc finger domain, 1B
• NCBI Official Symbol: BAZ1B
• NCBI Official Synonym Symbols: WSTF; WBSCR9; WBSCR10
• NCBI Protein Information: tyrosine-protein kinase BAZ1B; hWALp2; transcription factor WSTF; williams syndrome transcription factor; williams-Beuren syndrome chromosomal region 9 protein; williams-Beuren syndrome chromosomal region 10 protein
• UniProt Protein Name: Tyrosine-protein kinase BAZ1B
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: BAZ1B
• UniProt Synonym Gene Names: WBSC10; WBSCR10; WBSCR9; WSTF
• UniProt Entry Name: BAZ1B_HUMAN

NCBI Description

This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23. [provided by RefSeq, Jul 2008]

Uniprot Description

WSTF: plays a central role in chromatin remodeling and acts as a transcription regulator. Apparently possesses tyrosine-protein kinase activity, but bears no sequence resemblance classical tyrosine kinase proteins. Involved in DNA damage response by phosphorylating Y142 of histone H2AX. H2AXpY142 plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at Y142, and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association. Accumulates in pericentromeric heterochromatin during replication. Targeted to replication foci throughout S phase via its association with PCNA. Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary. Two alternatively-spliced human isoforms have been reported.

Protein type: Protein kinase, Ser/Thr (non-receptor); DNA replication; Kinase, protein; EC 2.7.10.2; Nuclear receptor co-regulator; ATYPICAL group; BAZ family

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: centric heterochromatin; nuclear replication fork; condensed chromosome

Molecular Function: protein binding; zinc ion binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; histone kinase activity; chromatin binding; ATP binding

Biological Process: chromatin assembly or disassembly; heart morphogenesis; peptidyl-tyrosine phosphorylation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; chromatin-mediated maintenance of transcription; histone phosphorylation; double-strand break repair; response to DNA damage stimulus