Sheep anti-Mouse Bax-beta Polyclonal Antibody | anti-Bax-beta antibody

Bax-beta

Cat. Num. AAA395786

• Gene Names: BAX; BCL2L4
• Reactivity: Mouse
• Applications: Immunoprecipitation
• Purity: Ammonium Sulfate Precipitation
• Synonyms: Bax-beta; Polyclonal Antibody; Apoptosis regulator Bax cytoplasmic isoform beta ; BaxB; BAX; BCL2 associated X protein; Apoptosis regulator BAX; BCL2 associated X protein isoform beta; Bax beta; Bax B; anti-Bax-beta antibody

• Host: Sheep
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Ammonium Sulfate Precipitation
• Form/Format: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Sequence Length: 218

• Applicable Applications for anti-Bax-beta antibody: Immunoprecipitation (IP)
• Immunogen: Synthetic peptide corresponding to amino acids 172 to 199 of theBax protein
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Western Blot/Blotting

(Immunoprecipitation of in)

Related Product Information for anti-Bax-beta antibody

Bax is encoded by a 1.5 kb RNA species created by the retention of intron 5 in the bax mRNA transcript. The exon4/intron5 splice junction is evolutionarily conserved and is found in both the human and mouse bax coding regions. The 630 bp intron 5 contributes 60 amino acids to the Bax protein before a stop codon is encountered during translation. The Bax protein is 24 kDa in size and although it contains the BH1, BH2, and BH3 domains identical to Bax, it has a unique carboxyl terminus and does not have a hydrophobic transmembrane domain (Oltvai et al., 1993). Bax mRNA has been found in many mouse tissues using RT-PCR. It is found co-expressed with Bax in lung, stomach, kidney, thymus, lymph node, bone marrow, pancreas, and spleen. Bax mRNA was very abundant in brain and duodenum with almost no Bax mRNA detected in these tissues (Oltvai et al., 1993).

Product Categories/Family for anti-Bax-beta antibody

Polyclonal Antibody; Bcl-2 Family

NCBI and Uniprot Product Information

• NCBI GI #: 4757838
• NCBI GeneID: 581
• NCBI Accession #: NP_004315.1
• NCBI GenBank Nucleotide #: NM_004324.3
• UniProt Accession #: Q07812; P55269; Q07814; Q07815; Q8WZ49; Q9NR76; Q9NYG7; Q9UCZ6; Q9UCZ7; Q9UQD6; A8K4W1
• Molecular Weight: 21 kDa
• NCBI Official Full Name: apoptosis regulator BAX isoform beta
• NCBI Official Synonym Full Names: BCL2-associated X protein
• NCBI Official Symbol: BAX
• NCBI Official Synonym Symbols: BCL2L4
• NCBI Protein Information: apoptosis regulator BAX; bcl2-L-4; bcl-2-like protein 4; BCL2-associated X protein omega; BCL2-associated X protein transcript variant delta2
• UniProt Protein Name: Apoptosis regulator BAX
• UniProt Gene Name: BAX
• UniProt Synonym Gene Names: BCL2L4; Bcl2-L-4
• UniProt Entry Name: BAX_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. Ref.1 Ref.4 Ref.12 Ref.18 Ref.25 Ref.26

Subunit structure: Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Interacts with human cytomegalovirus/HHV-5 protein vMIA/UL37. Interacts with BOP/C22orf29. Ref.1 Ref.4 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.22 Ref.23 Ref.25 Ref.26

Subcellular location: Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm Ref.1 Ref.13 Ref.16 Ref.18 Ref.23. Note: Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. Ref.1 Ref.13 Ref.16 Ref.18 Ref.23Isoform Beta: Cytoplasm Ref.1 Ref.13 Ref.16 Ref.18 Ref.23. Isoform Gamma: Cytoplasm Ref.1 Ref.13 Ref.16 Ref.18 Ref.23. Isoform Delta: Cytoplasm

Potential Ref.1 Ref.13 Ref.16 Ref.18 Ref.23.

Tissue specificity: Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. Ref.4 Ref.5

Domain: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family

By similarity.

Sequence similarities: Belongs to the Bcl-2 family.