Rabbit BAX Polyclonal Antibody | anti-BAX antibody

Rabbit anti BAX

Cat. Num. AAA225064

• Gene Names: BAX; BCL2L4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by affinity chromatography
• Synonyms: BAX; Polyclonal Antibody; Rabbit anti BAX; BAX Antibody; anti-BAX antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by affinity chromatography
• Form/Format: Liquid in phosphate buffered saline with 0.1% sodium azide (NaN3), 0.2% gelatin.
• Concentration: 0.2 mg/ml (varies by lot)
• Sequence Length: 192

• Applicable Applications for anti-BAX antibody: Western Blot (WB)
• Application Notes: WB: 1:400
• Immunogen: Peptide mapping to the amino terminus of human BAX
• Preperation: Antiserum to BAX was raised by repeated immunisation of rabbits with highly purified antigen.
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE.; This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch

Western Blot (WB)

(Western blotting analysis of HeLa whole cell lysate probed with Rabbit anti BAX antibody)

Related Product Information for anti-BAX antibody

Rabbit anti BAX antibody recognizes the Bcl-2-associated X protein (BAX) also known as Bcl-2 like protein 4. BAX together with BAK (BCL-2 antagonist or killer), another member of the Bcl-2 family, plays a key role in the intrinsic apoptosis pathway by inducing mitochondrial outer membrane permeabilization (MOMP) (Giansanti and Scovassi 2008). This event is aided by BID (BH3-interacting domain death agonist), which activates the two proteins and thereby facilitates the formation of homo- or heterodimers. Often in the literature, MOMP is regarded as the point of no return upon which a cell is committed to undergo apoptosis (Chipuk et al. 2006).

NCBI and Uniprot Product Information

• NCBI GI #: 728945
• NCBI GeneID: 581
• UniProt Accession #: Q07812; P55269; Q07814; Q07815; Q8WZ49; Q9NR76; Q9NYG7; Q9UCZ6; Q9UCZ7; Q9UQD6; A8K4W1
• Molecular Weight: 19,718 Da
• NCBI Official Full Name: Apoptosis regulator BAX
• NCBI Official Synonym Full Names: BCL2 associated X, apoptosis regulator
• NCBI Official Symbol: BAX
• NCBI Official Synonym Symbols: BCL2L4
• NCBI Protein Information: apoptosis regulator BAX
• UniProt Protein Name: Apoptosis regulator BAX
• Protein Family: Bax inhibitor
• UniProt Gene Name: BAX
• UniProt Synonym Gene Names: BCL2L4; Bcl2-L-4

NCBI Description

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.