Immunohistochemistry (IHC)
(MBS9609129 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human Basonuclin Polyclonal Antibody | anti-BNC1 antibody
Basonuclin Antibody
Gene Names
BNC1; BNC; BSN1; HsT19447
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Basonuclin; Polyclonal Antibody; Basonuclin Antibody; AI047752; AW546376; basonuclin 1; BNC; Bnc1; BNC1_HUMAN; BSN1; HsT19447; zinc finger protein basonuclin; Zinc finger protein basonuclin-1; anti-BNC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Basonuclin antibody detects endogenous levels of total Basonuclin
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
987
Applicable Applications for anti-BNC1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human Basonuclin
Subcellular Location
Nucleus. Cytoplasm. Relocates to the midpiece of the flagellum during late spermiogenesis in spermatids.
Tissue Specificity
In epidermis, primarily detected in cells of the basal or immediately suprabasal layers (at protein level) (PubMed:16891417). In hair follicles, mainly expressed in the outer root sheath (at protein level) (PubMed:8034748). Expressed in epidermis, testis and foreskin, and to a lower extent in thymus, spleen, mammary glands, placenta, brain and heart (PubMed:9687312).
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (83%), Bovine (92%), Horse (92%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9609129 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9609129 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of Basonuclin using HepG2 whole cell lysates)
Western Blot (WB)
(Western blot analysis of Basonuclin using HepG2 whole cell lysates)
Related Product Information for anti-BNC1 antibody
Function: Transcriptional activator (By similarity). Likely specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation (PubMed:8034748). Required for the maintenance of spermatogenesis (By similarity). May also play a role in the differentiation of oocytes and the early development of embryos (By similarity).
Subunit Structure: Interacts with HSF2BP (via C-terminus).
Post-translational Modifications: Phosphorylation on Ser-537 and Ser-541 leads to cytoplasmic localization.
Subunit Structure: Interacts with HSF2BP (via C-terminus).
Post-translational Modifications: Phosphorylation on Ser-537 and Ser-541 leads to cytoplasmic localization.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 111 kDa
Predicted: 111 kDa
Predicted: 111 kDa
NCBI Official Full Name
zinc finger protein basonuclin-1 isoform b
NCBI Official Synonym Full Names
basonuclin 1
NCBI Official Symbol
BNC1
NCBI Official Synonym Symbols
BNC; BSN1; HsT19447
NCBI Protein Information
zinc finger protein basonuclin-1
UniProt Protein Name
Zinc finger protein basonuclin-1
Protein Family
UniProt Gene Name
BNC1
UniProt Synonym Gene Names
BNC
NCBI Description
The protein encoded by this gene is a zinc finger protein present in the basal cell layer of the epidermis and in hair follicles. It is also found in abundance in the germ cells of testis and ovary. This protein is thought to play a regulatory role in keratinocyte proliferation and it may also be a regulator for rRNA transcription. Alternative splicing of this gene results in multiple transcript variants, and multiple polyadenylation sites are indicated.[provided by RefSeq, Jul 2014]
Uniprot Description
Transcriptional activator (). Likely specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation (PubMed:8034748). Required for the maintenance of spermatogenesis (). May also play a role in the differentiation of oocytes and the early development of embryos ().
Research Articles on BNC1
Similar Products
Product Notes
The BNC1 bnc1 (Catalog #AAA9609129) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Basonuclin Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Basonuclin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the BNC1 bnc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Basonuclin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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