Rabbit anti-Human BARON Polyclonal Antibody | anti-BARON antibody

BARON, NT (KIAA0226, Run domain Beclin-1 interacting and cystein-rich containing protein, Beclin-1 associated RUN domain containing protein) (MaxLight 405)

Cat. Num. AAA6277619

• Gene Names: RUBCN; SCAR15; RUBICON; KIAA0226
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BARON; Polyclonal Antibody; NT (KIAA0226; Run domain Beclin-1 interacting and cystein-rich containing protein; Beclin-1 associated RUN domain containing protein) (MaxLight 405); KIAA0226; Beclin-1 associated RUN domain containing protein; anti-BARON antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-BARON antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: BARON antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249~278 amino acids from the N-terminal region of human BARON.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-BARON antibody

MaxLight405 is a new Violet photostable dye conjugate comparable to Alexa Fluor 405, PacificBlue, Brilliant Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (400nm); Emission (423nm); Extinction Coefficient 32,000.

Product Categories/Family for anti-BARON antibody

Antibodies; KIAA; orf

NCBI and Uniprot Product Information

• NCBI GI #: 224548938
• NCBI GeneID: 9711
• NCBI Accession #: NP_001139114.1
• NCBI GenBank Nucleotide #: NM_001145642.4
• UniProt Accession #: Q92622; Q96CK5
• Molecular Weight: 102,506 Da
• NCBI Official Full Name: run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform 1
• NCBI Official Synonym Full Names: RUN and cysteine rich domain containing beclin 1 interacting protein
• NCBI Official Symbol: RUBCN
• NCBI Official Synonym Symbols: SCAR15; RUBICON; KIAA0226
• NCBI Protein Information: run domain Beclin-1-interacting and cysteine-rich domain-containing protein
• UniProt Protein Name: Run domain Beclin-1-interacting and cysteine-rich domain-containing protein
• UniProt Gene Name: RUBCN
• UniProt Synonym Gene Names: Rubicon; Baron

NCBI Description

The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

Uniprot Description

Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation (PubMed:20974968, PubMed:21062745).