Rabbit anti-Human, Mouse BAP31 Polyclonal Antibody | anti-BAP31 antibody

BAP31 Polyclonal Antibody

Cat. Num. AAA3012291

• Gene Names: BCAP31; CDM; DDCH; BAP31; 6C6-AG; DXS1357E
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: BAP31; Polyclonal Antibody; BAP31 Polyclonal Antibody; BCR-associated protein 31; Bap31; BCAP31; DXS1357E; 6C6 AG; 6C6 AG tumor associated antigen; 6C6-AG tumor-associated antigen; 6C6AG; 6C6AG tumor associated antigen; Accessory protein BAP 31; Accessory protein BAP31; B cell receptor associated protein 31; B-cell receptor-associated protein 31; BA31; BAP 31; BCAP 31; BCR associated protein Bap 31; BCR associated protein Bap31; CDM; CDM protein; MS950; p28; p28 Bap31; Protein CDM; RP23-329M9.5; anti-BAP31 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BAP31 polyclonal antibody detects endogenous levels of BAP31 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 246

• Applicable Applications for anti-BAP31 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:2,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: Synthetic peptide within the C-terminus of human BAP31.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of BAP31 on different lysates using anti-BAP31 antibody at 1/1,000 dilution. Positive control:Lane 1: A431 Lane 2: SK-Br-3)

Immunocytochemistry (ICC)

(ICC staining BAP31 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-BAP31 antibody

BAP31, a human Bcl-2-interacting protein, is an integral membrane protein that is a component of a protein complex in the endoplasmic reticulum. This protein complex mechanically bridges an apoptosis-initiating caspase, like procaspase-8, with the anti-apoptotic regulator Bcl-2 or Bcl-XL. The cytosolic domain of BAP31 contains two identical caspase recognition sites, which are preferentially cleaved by initiator caspases, including caspase 8. Cleavage of BAP31 during apoptosis generates a p20 fragment, which remains integrated in the membrane and, when expressed ectopically, is a potent inducer of cell death. BAP31 cleavage is important for manifesting cytoplasmic apoptotic events associated with membrane fragmentation and in the cross talk between mitochondria and the endoplasmic reticulum during Fas- mediated apoptosis. The BAP31 gene is ubiquitously expressed in murine tissues and is located on the X chromosome in both mouse and human.

NCBI and Uniprot Product Information

• NCBI GI #: 213511012
• NCBI GeneID: 10134
• NCBI Accession #: NP_001132913.1
• NCBI GenBank Nucleotide #: NP_001132913.1
• UniProt Accession #: P51572; Q61335
• Molecular Weight: 28kDa
• NCBI Official Full Name: B-cell receptor-associated protein 31 isoform b
• NCBI Official Synonym Full Names: B cell receptor associated protein 31
• NCBI Official Symbol: BCAP31
• NCBI Official Synonym Symbols: CDM; DDCH; BAP31; 6C6-AG; DXS1357E
• NCBI Protein Information: B-cell receptor-associated protein 31
• UniProt Protein Name: B-cell receptor-associated protein 31
• UniProt Gene Name: BCAP31
• UniProt Synonym Gene Names: BAP31; DXS1357E; BCR-associated protein 31; Bap31
• UniProt Entry Name: BAP31_HUMAN

NCBI Description

This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16. [provided by RefSeq, Jan 2012]

Uniprot Description

BCAP31 iso2: May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis. Homodimer and heterodimer with BCAP29. Binds CASP8 (isoform 9) as a complex containing BCAP31, BCAP29, BCL2 and/or BCL2L1. Interacts with VAMP3, VAMP1 and membrane IgD immunoglobulins. May interact with ACTG1 and non-muscle myosin II. Interacts with PTPLB. Ubiquitous. Belongs to the BCAP29/BCAP31 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; clathrin-coated vesicle; membrane; endoplasmic reticulum; integral to plasma membrane; lipid particle; cytosol

Molecular Function: protein binding; MHC class I protein binding; protein complex binding

Biological Process: intracellular protein transport; elevation of cytosolic calcium ion concentration; antigen processing and presentation of peptide antigen via MHC class I; ER to Golgi vesicle-mediated transport; apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of caspase activity; spermatogenesis; elevation of mitochondrial calcium ion concentration; cell structure disassembly during apoptosis

Disease: Deafness, Dystonia, And Cerebral Hypomyelination