Rabbit anti-Human BANF1 Polyclonal Antibody | anti-BANF1 antibody

BANF1, ID (BANF1, BAF, BCRG1, Barrier-to-autointegration factor, Breakpoint cluster region protein 1) (MaxLight 550)

Cat. Num. AAA6277588

• Gene Names: BANF1; BAF; NGPS; BCRP1; D14S1460
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: BANF1; Polyclonal Antibody; ID (BANF1; BAF; BCRG1; Barrier-to-autointegration factor; Breakpoint cluster region protein 1) (MaxLight 550); Breakpoint cluster region protein 1; anti-BANF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BANF1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-BANF1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 37-65 in the central region of human BANF1, UniProt Accession #O75531. Species sequence homology: bovine, mouse, rat
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS6001966. This demonstrates that MBS6001966 detected the BANF1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue using MBS6001966 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Product Categories/Family for anti-BANF1 antibody

Antibodies; Proteins

References

1. Montes de Oca, R., et al. PLoS ONE 4 (9), E7050 (2009). 2. Haraguchi, T., et al. J. Cell. Sci. 121 (PT 15), 2540-2554 (2008). 3. Haraguchi, T., et al. J. Cell. Sci. 120 (PT 12), 1967-1977 (2007). 4. Wiebe, M.S., et al. Cell Host Microbe 1(3):187-197(2007). 5. Bengtsson, L., et al. Mol. Biol. Cell 17(3):1154-1163(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 221316573
• NCBI GeneID: 8815
• NCBI Accession #: NP_001137457.1; NP_003851.1
• NCBI GenBank Nucleotide #: NM_001143985.1
• UniProt Accession #: O75531; O60558; Q6FGG7
• Molecular Weight: 10,059 Da
• NCBI Official Full Name: barrier-to-autointegration factor
• NCBI Official Synonym Full Names: barrier to autointegration factor 1
• NCBI Official Symbol: BANF1
• NCBI Official Synonym Symbols: BAF; NGPS; BCRP1; D14S1460
• NCBI Protein Information: barrier-to-autointegration factor; breakpoint cluster region protein 1
• UniProt Protein Name: Barrier-to-autointegration factor
• Protein Family: Barrier-to-autointegration factor
• UniProt Gene Name: BANF1
• UniProt Synonym Gene Names: BAF; BCRG1
• UniProt Entry Name: BAF_HUMAN

Uniprot Description

BAF: Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self- destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Defects in BANF1 are the cause of Nestor-Guillermo progeria syndrome (NGPS). NGPS is an atypical progeroid syndrome characterized by normal development in the first years of life, later followed by the emergence of generalized lipoatrophy, severe osteoporosis, and marked osteolysis. The atrophic facial subcutaneous fat pad and the marked osteolysis of the maxilla and mandible result in a typical pseudosenile facial appearance with micrognatia, prominent subcutaneous venous patterning, a convex nasal ridge, and proptosis. Cognitive development is completely normal. Patients do not have cardiovascular dysfunction, atherosclerosis, or metabolic anomalies. Belongs to the BAF family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: nucleoplasm; cytoplasm; chromosome; nucleus; cytosol

Molecular Function: protein binding; DNA binding

Biological Process: mitotic nuclear envelope reassembly; viral reproduction; DNA integration; response to virus; mitotic nuclear envelope disassembly; mitotic cell cycle

Disease: Nestor-guillermo Progeria Syndrome