Rabbit anti-Human Bak Polyclonal Antibody | anti-Bak antibody

Bak, BH3 Domain (Bcl-2 Homologous Antagonist/Killer, Apoptosis Regulator BAK, Bcl-2-like Protein 7, Bcl2-L-7, BAK1, BCL2L7, CDN1) (MaxLight 650)

Cat. Num. AAA6464910

• Gene Names: BAK1; BAK; CDN1; BCL2L7; BAK-LIKE
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Bak; Polyclonal Antibody; BH3 Domain (Bcl-2 Homologous Antagonist/Killer; Apoptosis Regulator BAK; Bcl-2-like Protein 7; Bcl2-L-7; BAK1; BCL2L7; CDN1) (MaxLight 650); anti-Bak antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Bak.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-Bak antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 56-91 of human Bak, UniProt Accession #NP_001179; Q16611.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS623426, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western Blot analysis in Jurkat, MDA-MB453, 293 cell line lysates (35ug/lane) usingMBS623426. This demonstrates that MBS623426 detected the Bak protein (arrow).)

Product Categories/Family for anti-Bak antibody

Antibodies; Apoptosis

References

1. Cartron, P.F., et al., Mol. Cell. Biol. 23(13):4701-4712 (2003). 2. Mikhailov, V., et al., J. Biol. Chem. 278(7):5367-5376 (2003). 3. Werner, A.B., et al., J. Biol. Chem. 277(25):22781-22788 (2002). 4. Bellosillo, B., et al., Blood 100(5):1810-1816 (2002). 5. Grutkoski, P.S., et al., Shock 17(1):47-54 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4502363
• NCBI GeneID: 578
• NCBI Accession #: NP_001179
• NCBI GenBank Nucleotide #: NM_001188.3
• UniProt Accession #: Q16611; Q6I9T6; Q92533; C0H5Y7
• Molecular Weight: 16,872 Da
• NCBI Official Full Name: bcl-2 homologous antagonist/killer
• NCBI Official Synonym Full Names: BCL2 antagonist/killer 1
• NCBI Official Symbol: BAK1
• NCBI Official Synonym Symbols: BAK; CDN1; BCL2L7; BAK-LIKE
• NCBI Protein Information: bcl-2 homologous antagonist/killer
• UniProt Protein Name: Bcl-2 homologous antagonist/killer
• UniProt Gene Name: BAK1
• UniProt Synonym Gene Names: BAK; BCL2L7; CDN1; Bcl2-L-7
• UniProt Entry Name: BAK_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008]

Uniprot Description

BAK1: In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. Interacts with BCL2A1. Homodimer. Formation of the homodimer is zinc-dependent. Forms heterodimers with BCL2, E1B 19k protein, and BCL2L1 isoform Bcl-X(L). Interacts with myxoma virus protein M11L. Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. Belongs to the Bcl-2 family.

Protein type: Membrane protein, integral; Apoptosis; Endoplasmic reticulum; Mitochondrial

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: pore complex; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; cytosol; integral to mitochondrial outer membrane

Molecular Function: identical protein binding; BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity; chaperone binding; heat shock protein binding; metal ion binding

Biological Process: response to fungus; regulation of protein heterodimerization activity; positive regulation of apoptosis; apoptosis; regulation of cell cycle; positive regulation of proteolysis; myeloid cell homeostasis; negative regulation of peptidyl-serine phosphorylation; B cell apoptosis; response to organic cyclic substance; negative regulation of cell proliferation; B cell homeostasis; regulation of mitochondrial membrane potential; response to gamma radiation; establishment and/or maintenance of transmembrane electrochemical gradient; B cell negative selection; aging; response to drug; release of cytochrome c from mitochondria; organ regeneration; mitochondrial fusion; response to mycotoxin; regulation of protein homodimerization activity; vagina development; endocrine pancreas development; limb morphogenesis; response to UV-C; cell proliferation; endoplasmic reticulum calcium ion homeostasis; response to ethanol; unfolded protein response, activation of signaling protein activity; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; reduction of endoplasmic reticulum calcium ion concentration; blood vessel remodeling; caspase activation via cytochrome c; brain development; regulation of mitochondrial membrane permeability; post-embryonic camera-type eye morphogenesis