Rabbit anti-Mouse BAD Polyclonal Antibody | anti-BAD antibody

BAD, Phosphorylated (S112) (Bad, Bbc6, Bcl2 antagonist of cell death, Bcl-2-binding component 6, Bcl-xL/Bcl-2-associated death promoter) (PE)

Cat. Num. AAA6277327

• Gene Names: Bad; Bbc2; AI325008
• Reactivity: Mouse
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BAD; Polyclonal Antibody; Phosphorylated (S112) (Bad; Bbc6; Bcl2 antagonist of cell death; Bcl-2-binding component 6; Bcl-xL/Bcl-2-associated death promoter) (PE); Bad; Bcl-xL/Bcl-2-associated death promoter; anti-BAD antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-BAD antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Phospho-mouse BAD-S112 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S112 of mouse BAD.
• Conjugate: PE
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of Phospho-mouse BAD-S112 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-BAD antibody

BAD promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Product Categories/Family for anti-BAD antibody

Antibodies; Apoptosis

References

Santidrian, A.F., et al. Blood 116(16):3023-3032(2010). Frenzel, A., et al. Blood 115(5):995-1005(2010). Quoyer, J., et al. J. Biol. Chem. 285(3):1989-2002(2010). Polzien, L., et al. J. Biol. Chem. 284(41):28004-28020(2009). Wu, X., et al. Diabetologia 52(10):2130-2141(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 6671610
• NCBI GeneID: 12015
• NCBI Accession #: NP_031548.1
• NCBI GenBank Nucleotide #: NM_007522.2
• UniProt Accession #: Q61337
• Molecular Weight: 22,080 Da
• NCBI Official Full Name: bcl2 antagonist of cell death
• NCBI Official Synonym Full Names: BCL2-associated agonist of cell death
• NCBI Official Symbol: Bad
• NCBI Official Synonym Symbols: Bbc2; AI325008
• NCBI Protein Information: bcl2 antagonist of cell death; bcl-2-binding component 6; Bcl-associated death promoter; bcl-xL/Bcl-2-associated death promoter
• UniProt Protein Name: Bcl2 antagonist of cell death
• Protein Family: Bcl2-associated agonist of cell death
• UniProt Gene Name: Bad
• UniProt Synonym Gene Names: Bbc6; BAD
• UniProt Entry Name: BAD_MOUSE

Uniprot Description

BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL.

Protein type: Apoptosis

Cellular Component: mitochondrial outer membrane; membrane; mitochondrion; cytoplasm; intracellular; cytosol

Molecular Function: protein kinase B binding; protein binding; protein heterodimerization activity; phospholipid binding; caspase activator activity; protein phosphatase binding; lipid binding; protein kinase binding; protein phosphatase 2B binding

Biological Process: apoptosis; positive regulation of proteolysis; positive regulation of apoptosis; positive regulation of caspase activity; regulation of caspase activity; cellular process regulating host cell cycle in response to virus; glucose homeostasis; positive regulation of apoptosis by virus; regulation of apoptosis; pore complex biogenesis; positive regulation of glucokinase activity; caspase activation; release of cytochrome c from mitochondria; cytokine and chemokine mediated signaling pathway; suppression by virus of host apoptosis; ADP metabolic process; positive regulation of insulin secretion; ATP metabolic process; cell proliferation; glucose catabolic process; induction of apoptosis via death domain receptors; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of T cell differentiation; positive regulation of B cell differentiation; regulation of mitochondrial membrane permeability; positive regulation of epithelial cell proliferation