Rabbit BACE2, Asp1 Polyclonal Antibody | anti-BACE2 antibody

BACE2, Asp1 (CT) (Beta-site APP Cleaving Enzyme, Aspartyl Protease 2, DRAP, Down Region Aspartic Protease, Memapsin)

Cat. Num. AAA617722

• Gene Names: BACE2; ASP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Immunoaffinity chromatography purified IgG
• Synonyms: BACE2; Asp1; Polyclonal Antibody; Asp1 (CT) (Beta-site APP Cleaving Enzyme; Aspartyl Protease 2; DRAP; Down Region Aspartic Protease; Memapsin); Anti -BACE2; anti-BACE2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BACE2. Species crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Immunoaffinity chromatography purified IgG
• Form/Format: Supplied as a liquid, 100ug in 200ul of PBS, 0.02% sodium azide.

• Applicable Applications for anti-BACE2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Western Blotting.; Dilution: Western Blotting: 0.5-1ug/ml.
• Immunogen: Raised against a peptide corresponding to amino acids 496 to 511 of human BACE2 (4).
• Positive Control: Human heart tissue lysate can be used as positive control.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemicalanalysis of MBS617722.)

Related Product Information for anti-BACE2 antibody

Accumulation of the amyloid-b (Ab) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Ab peptide is generated by proteolytic cleavage of the b-amyloid protein precursor (APP) at b-and g-sites by proteases. The long-sought b-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2) (1-4). A BACE homolog was recently cloned and designated BACE2, Asp1, DRAP (for Down region aspartic protease), and memapsin 1 (4-9). BACE2 also cleaves APP at b-site and at a different site within Ab (8). BACE2 locates on chromosome 21q22.3, the so-called 'Down critical region', suggesting that BACE2 and Ab may also contribute to the pathogenesis of Down syndrome (6,7)

Product Categories/Family for anti-BACE2 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 19923395
• NCBI GeneID: 25825
• NCBI Accession #: NP_036237.2
• NCBI GenBank Nucleotide #: NM_012105.4
• UniProt Accession #: Q9Y5Z0; Q5DIH8; Q8N2D4; Q9H2V8; Q9NZL1; Q9NZL2; Q9UJT6; A8K7P1
• Molecular Weight: 56,180 Da
• NCBI Official Full Name: beta-secretase 2 isoform A preproprotein
• NCBI Official Synonym Full Names: beta-site APP-cleaving enzyme 2
• NCBI Official Symbol: BACE2
• NCBI Official Synonym Symbols: ASP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1
• NCBI Protein Information: beta-secretase 2; memapsin-1; theta-secretase; aspartyl protease 1; 56 kDa aspartic-like protease; Down syndrome region aspartic protease; transmembrane aspartic proteinase Asp1; membrane-associated aspartic protease 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme 2
• UniProt Protein Name: Beta-secretase 2
• Protein Family: Beta-secretase
• UniProt Gene Name: BACE2
• UniProt Synonym Gene Names: AEPLC; ALP56; ASP21; ASP1; Asp 1; Beta-site APP cleaving enzyme 2; DRAP
• UniProt Entry Name: BACE2_HUMAN

NCBI Description

This gene encodes an integral membrane glycoprotein that functions as an aspartic protease. The encoded protein cleaves amyloid precursor protein into amyloid beta peptide, which is a critical step in the etiology of Alzheimer's disease and Down syndrome. The protein precursor is further processed into an active mature peptide. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

BACE2: Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C- terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672. Belongs to the peptidase A1 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.4.23.45; Protease

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: Golgi apparatus; cell surface; endoplasmic reticulum; integral to membrane; endosome

Molecular Function: aspartic-type endopeptidase activity

Biological Process: negative regulation of amyloid precursor protein biosynthetic process; peptide hormone processing; membrane protein ectodomain proteolysis; proteolysis