Rabbit anti-Human BACE2 Polyclonal Antibody | anti-BACE2 antibody

BACE2 Antibody

Cat. Num. AAA150389

• Gene Names: BACE2; ASP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: BACE2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: BACE2; Polyclonal Antibody; BACE2 Antibody; ASP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1; UNQ418/PRO852; Beta-secretase 2; Aspartic-like protease 56 kDa; beta-site APP-cleaving enzyme 2; anti-BACE2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: BACE2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 518

• Applicable Applications for anti-BACE2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: BACE2 antibody can be used for detection of BACE2 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: BACE2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human BACE2.
• Buffer: BACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: BACE2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of BACE2 in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide with BACE2 antibody at 1 μg/mL. )

Immunohistochemistry (IHC)

(Immunohistochemistry of BACE2 in rat heart tissue with BACE2 antibody at 2 μg/mL.)

Related Product Information for anti-BACE2 antibody

BACE2 Antibody: Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by proteases. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE/Asp2 is a novel transmembrane aspartic protease and co-localizes with APP. A BACE homolog was recently cloned and designated BACE2, Asp1, DRAP (for Down region aspartic protease), and memapsin 1. BACE2 also cleaves APP at b-site and at a different site within Abeta. BACE2 locates on chromosome 21q22.3, the so-called 'Down critical region', suggesting that BACE2 and Abeta may also contribute to the pathogenesis of Down syndrome.

NCBI and Uniprot Product Information

• NCBI GI #: 6561812
• NCBI GeneID: 25825
• UniProt Accession #: Q9Y5Z0; Q5DIH8; Q8N2D4; Q9H2V8; Q9NZL1; Q9NZL2; Q9UJT6; A8K7P1
• Molecular Weight: 46,476 Da
• NCBI Official Full Name: aspartyl protease 1
• NCBI Official Synonym Full Names: beta-site APP-cleaving enzyme 2
• NCBI Official Symbol: BACE2
• NCBI Official Synonym Symbols: ASP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1
• NCBI Protein Information: beta-secretase 2; memapsin-1; theta-secretase; aspartyl protease 1; 56 kDa aspartic-like protease; Down syndrome region aspartic protease; transmembrane aspartic proteinase Asp1; membrane-associated aspartic protease 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme 2
• UniProt Protein Name: Beta-secretase 2
• Protein Family: Beta-secretase
• UniProt Gene Name: BACE2
• UniProt Synonym Gene Names: AEPLC; ALP56; ASP21; ASP1; Asp 1; Beta-site APP cleaving enzyme 2; DRAP
• UniProt Entry Name: BACE2_HUMAN

NCBI Description

This gene encodes an integral membrane glycoprotein that functions as an aspartic protease. The encoded protein cleaves amyloid precursor protein into amyloid beta peptide, which is a critical step in the etiology of Alzheimer's disease and Down syndrome. The protein precursor is further processed into an active mature peptide. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

BACE2: Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C- terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672. Belongs to the peptidase A1 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.4.23.45; Protease

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: Golgi apparatus; cell surface; endoplasmic reticulum; integral to membrane; endosome

Molecular Function: aspartic-type endopeptidase activity

Biological Process: negative regulation of amyloid precursor protein biosynthetic process; peptide hormone processing; membrane protein ectodomain proteolysis; proteolysis