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Typical Testing Data/Standard Curve (for reference only)

Human VIP peptides ELISA Kit | VIP elisa kit

Human VIP peptides ELISA Kit

Gene Names
VIP; PHM27
Reactivity
Human
Synonyms
VIP peptides; Human VIP peptides ELISA Kit; VIP (vasoactive intestinal peptide)/VIP peptides/PHM27; VIP elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of VIP . No significant crossreactivity or interference between VIP and analogues was observed.
Sequence Length
169
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.313-20pg/ml
Sensitivity
<4.688pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VIP were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VIP were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for VIP elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- VIP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- VIP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the VIP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of VIP can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,082 Da
NCBI Official Full Name
VIP peptides isoform 2 preproprotein
NCBI Official Synonym Full Names
vasoactive intestinal peptide
NCBI Official Symbol
VIP
NCBI Official Synonym Symbols
PHM27
NCBI Protein Information
VIP peptides
UniProt Protein Name
VIP peptides
Protein Family
UniProt Gene Name
VIP
UniProt Synonym Gene Names
VIP
UniProt Entry Name
VIP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2014]

Uniprot Description

VIP: VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder. Belongs to the glucagon family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6q25

Cellular Component: cell; cell soma; extracellular region

Molecular Function: hormone activity; neuropeptide hormone activity; protein binding

Biological Process: antibacterial humoral response; antifungal humoral response; body fluid secretion; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; G-protein coupled receptor protein signaling pathway; innate immune response; learning and/or memory; mRNA stabilization; negative regulation of apoptosis; negative regulation of potassium ion transport; negative regulation of smooth muscle cell proliferation; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; positive regulation of protein catabolic process; positive regulation of vasodilation; regulation of protein localization; regulation of sensory perception of pain; response to yeast

Research Articles on VIP

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Product Notes

The Human VIP vip (Catalog #AAA765423) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA765423 ELISA Kit recognizes Human VIP. It is sometimes possible for the material contained within the vial of "VIP peptides, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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