Rabbit anti-Human, Mouse B4GALT6 Polyclonal Antibody | anti-B4GALT6 antibody

B4GALT6 Polyclonal Antibody

Cat. Num. AAA3006939

• Gene Names: B4GALT6; B4Gal-T6; beta4Gal-T6
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: B4GALT6; Polyclonal Antibody; B4GALT6 Polyclonal Antibody; Beta-1; 4-galactosyltransferase 6; 4-GalTase 6; Beta4Gal-T6; b4Gal-T6; UDP-Gal: beta-GlcNAc beta-1; UDP-galactose: beta-N-acetylglucosamine beta-1; Including the following 1 domains: Glucosylceramide beta-1; 4-galactosyltransferase; Lactosylceramide synthase; LacCer synthase; UDP-Gal: glucosylceramide beta-1; anti-B4GALT6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: B4GALT6 polyclonal antibody detects endogenous levels of B4GALT6 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 343

• Applicable Applications for anti-B4GALT6 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human B4GALT6
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of B4GALT6 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:A549 whole cell lysate Lane3:sp2/0 whole cell lysate)

Related Product Information for anti-B4GALT6 antibody

beta-1, 4-galactosyltransferases (beta-1, 4-Gal-T) are type II membrane-bound glycoproteins that are substrate-specific and function to transfer galactose in a beta-1, 4 linkage to an acceptor sugar. There are seven members of the beta-1, 4-Gal-T family, all of which are directed to the Golgi apparatus through a hydrophobic sequence at the N-terminus. beta-1, 4-Gal-T6, also known as B4GALT6, is a 382 amino acid single-pass type II membrane protein that localizes to the Golgi apparatus and belongs to the beta-1, 4-galactosyltransferase family. Expressed at high levels in adrenal gland and brain and present at lower levels in lung, liver and colon, beta-1, 4-Gal-T6 uses magnesium or zinc to catalyze the UDP-dependent biosynthesis of glycosphingolipids. The gene encoding beta-1, 4-Gal-T6 maps to human chromosome 18, which houses over 300 protein-coding genes and contains nearly 76 million bases.

NCBI and Uniprot Product Information

• NCBI GI #: 46854414
• NCBI GeneID: 9331
• UniProt Accession #: Q9UBX8
• Molecular Weight: ~ 45kDa
• NCBI Official Full Name: B4GALT6 protein
• NCBI Official Synonym Full Names: beta-1,4-galactosyltransferase 6
• NCBI Official Symbol: B4GALT6
• NCBI Official Synonym Symbols: B4Gal-T6; beta4Gal-T6
• NCBI Protein Information: beta-1,4-galactosyltransferase 6
• UniProt Protein Name: Beta-1,4-galactosyltransferase 6
• Protein Family: Beta-1,4-galactosyltransferase
• UniProt Gene Name: B4GALT6
• UniProt Synonym Gene Names: Beta-1,4-GalTase 6; Beta4Gal-T6; b4Gal-T6; LacCer synthase
• UniProt Entry Name: B4GT6_HUMAN

NCBI Description

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes in human. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. This gene produces multiple protein isoforms - some of which are predicted to lack the N-terminal hydrophobic signal sequence and transmembrane domain. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The canonical enzyme encoded by this gene is a lactosylceramide synthase important for glycolipid biosynthesis. [provided by RefSeq, Jan 2020]

Uniprot Description

Function: Required for the biosynthesis of glycosphingolipids. Ref.5 Ref.6

Catalytic activity: UDP-galactose + glucosylceramide = UDP + lactosylceramide. Ref.2 Ref.5 Ref.6UDP-alpha-D-galactose + beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide. Ref.2 Ref.5 Ref.6

Cofactor: Manganese or magnesium. Ref.5

Enzyme regulation: Inhibited by EDTA

By similarity.

Pathway: Protein modification; protein glycosylation.

Subcellular location: Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Trans cisternae of Golgi stack.

Tissue specificity: High expression in brain and adrenal gland, lower in liver, lung, colon and peripheral white blood cells.

Sequence similarities: Belongs to the glycosyltransferase 7 family.

Biophysicochemical propertiesKinetic parameters:KM=3 µM for glucosylceramide Ref.5KM=0.5 µM for UDP-galactoseVmax=0.06 nmol/h/mg enzyme toward glucosylceramideVmax=86 nmol/h/mg enzyme toward UDP-galactose