Rabbit B4GALT6 Polyclonal Antibody | anti-B4GALT6 antibody

B4GALT6 Antibody (C-term)

Cat. Num. AAA9210579

• Gene Names: B4GALT6; B4Gal-T6; beta4Gal-T6
• Reactivity: Human (Predicted Reactivity: Mouse)
• Applications: ELISA, Immunohistochemistry, Flow Cytometry, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: B4GALT6; Polyclonal Antibody; B4GALT6 Antibody (C-term); Beta-1; 4-galactosyltransferase 6; 4-GalTase 6; Beta4Gal-T6; b4Gal-T6; 241-; UDP-Gal:beta-GlcNAc beta-1; UDP-galactose:beta-N-acetylglucosamine beta-1; Glucosylceramide beta-1; 4-galactosyltransferase; Lactosylceramide synthase; LacCer synthase; UDP-Gal:glucosylceramide beta-1; anti-B4GALT6 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This B4GALT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-346 amino acids from the C-terminal region of human B4GALT6.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 319-346
• Sequence Length: 382

• Applicable Applications for anti-B4GALT6 antibody: ELISA (EIA), Immunohistochemistry-parrafin (IHC-p), Flow Cytometry (FC), Western Blot (WB)
• Application Notes: WB~~1:1000; IHC-P~~1:50~100; FC~~1:10~50
• Cellular Location: Golgi apparatus, Golgi stack memrane; Single-pass type II membrane protein. Note= Trans cisternae of Golgi stack.
• Tissue Location: High expression in brain and adrenal gland, lower in liver, lung, colon and peripheral white blood cells.
• Antigen Region: 319-346
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(B4GALT6 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the B4GALT6 antibody detected the B4GALT6 protein (arrow).)

Immunohistochemistry (IHC)

(B4GALT6 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the B4GALT6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(B4GALT6 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-B4GALT6 antibody

Function Catalyzes the synthesis of lactosylceramide (LacCer) via the transfer of galactose from UDP-galactose to glucosylceramide (GlcCer) (PubMed:3099851, PubMed:1551920, PubMed:24498430). LacCer is the starting point in the biosynthesis of all gangliosides (membrane-bound glycosphingolipids) which play pivotal roles in the CNS including neuronal maturation and axonal and myelin formation (By similarity)

Product Categories/Family for anti-B4GALT6 antibody

Metabolism; Signal Transduction

References

Landers, J.E., et al. Proc. Natl. Acad. Sci. U.S.A. 106(22):9004-9009(2009) Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003) Fan, Y., et al. DNA Seq. 13(1):1-8(2002) Amado, M., et al. Biochim. Biophys. Acta 1473(1):35-53(1999) Takizawa, M., et al. Biochim. Biophys. Acta 1438(2):301-304(1999)

NCBI and Uniprot Product Information

• NCBI GI #: 190570165
• NCBI GeneID: 9331
• NCBI Accession #: NP_004766.2
• NCBI GenBank Nucleotide #: NM_004775.3
• UniProt Accession #: Q9UBX8; O60514; Q6NT09
• Molecular Weight: 44914
• NCBI Official Full Name: beta-1,4-galactosyltransferase 6
• NCBI Official Synonym Full Names: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 6
• NCBI Official Symbol: B4GALT6
• NCBI Official Synonym Symbols: B4Gal-T6; beta4Gal-T6
• NCBI Protein Information: beta-1,4-galactosyltransferase 6
• UniProt Protein Name: Beta-1,4-galactosyltransferase 6
• Protein Family: Beta-1,4-galactosyltransferase
• UniProt Gene Name: B4GALT6
• UniProt Synonym Gene Names: Beta-1,4-GalTase 6; Beta4Gal-T6; b4Gal-T6; LacCer synthase
• UniProt Entry Name: B4GT6_HUMAN

NCBI Description

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene is a lactosylceramide synthase important for glycolipid biosynthesis. [provided by RefSeq, Jul 2008]

Uniprot Description

B4GALT6: Required for the biosynthesis of glycosphingolipids. Belongs to the glycosyltransferase 7 family.

Protein type: Transferase; EC 2.4.1.274; Lipid Metabolism - sphingolipid; Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q11

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: metal ion binding; galactosyltransferase activity

Biological Process: keratan sulfate metabolic process; cellular protein metabolic process; glycosaminoglycan metabolic process; keratan sulfate biosynthetic process; carbohydrate metabolic process; pathogenesis; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification