Rabbit anti-Human B4GALT2 Polyclonal Antibody | anti-B4GALT2 antibody

B4GALT2 Antibody

Cat. Num. AAA7051777

• Gene Names: B4GALT2; B4Gal-T2; B4Gal-T3; beta4Gal-T2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: >95%, Protein G purified
• Synonyms: B4GALT2; Polyclonal Antibody; B4GALT2 Antibody; Beta-1; 4-galactosyltransferase 2; 4-GalTase 2; Beta4Gal-T2; b4Gal-T2; UDP-Gal:beta-GlcNAc beta-1; UDP-galactose:beta-N-acetylglucosamine beta-1; Lactose synthase A protein; N-acetyllactosamine synthase; Nal synthase; Beta-N-acetylglucosaminylglycopeptide beta-1; 4-galactosyltransferase; Beta-N-acetylglucosaminyl-glycolipid beta-1; anti-B4GALT2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 372

• Applicable Applications for anti-B4GALT2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:500-1:5000
• Immunogen: Recombinant Human Beta-1, 4-galactosyltransferase 2 protein (271-372AA)
• Conjugation: Non-conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: A549 whole cell lysate,HepG2 whole cell lysate,U251 whole cell lysateAll lanes: B4GALT2 antibody at 3.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42,35,45 KDaObserved band size: 42 KDa)

Related Product Information for anti-B4GALT2 antibody

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose.

NCBI and Uniprot Product Information

• NCBI GI #: 53759113
• NCBI GeneID: 8704
• NCBI Accession #: NP_001005417.1
• NCBI GenBank Nucleotide #: NM_001005417.2
• UniProt Accession #: O60909; O60511; Q4V9L9; Q5T4X5; Q5T4Y5; Q9BUP6; Q9NSY7; B3KTP0; B4DE14; D3DPY6; D3DPY7
• Molecular Weight: 44,764 Da
• NCBI Official Full Name: beta-1,4-galactosyltransferase 2 isoform b
• NCBI Official Synonym Full Names: beta-1,4-galactosyltransferase 2
• NCBI Official Symbol: B4GALT2
• NCBI Official Synonym Symbols: B4Gal-T2; B4Gal-T3; beta4Gal-T2
• NCBI Protein Information: beta-1,4-galactosyltransferase 2
• UniProt Protein Name: Beta-1,4-galactosyltransferase 2
• Protein Family: Beta-1,4-galactosyltransferase
• UniProt Gene Name: B4GALT2
• UniProt Synonym Gene Names: Beta-1,4-GalTase 2; Beta4Gal-T2; b4Gal-T2

NCBI Description

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene synthesizes N-acetyllactosamine in glycolipids and glycoproteins. Its substrate specificity is affected by alpha-lactalbumin but it is not expressed in lactating mammary tissue. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

B4GALT2: Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose. Belongs to the glycosyltransferase 7 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - galactose; EC 2.4.1.22; EC 2.4.1.38; EC 2.4.1.90; Glycan Metabolism - N-glycan biosynthesis; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Glycan Metabolism - keratan sulfate biosynthesis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: Golgi membrane

Molecular Function: beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity; galactosyltransferase activity

Biological Process: keratan sulfate biosynthetic process