Western Blot (WB)
(B3GNT4 Antaibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the B3GNT4 antibody detected the B3GNT4 protein (arrow).)
Rabbit anti-Human, Mouse B3GNT4 Polyclonal Antibody | anti-B3GNT4 antibody
B3GNT4, ID (B3GNT4, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4) (APC)
Gene Names
B3GNT4; B3GN-T4; beta3Gn-T4
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
B3GNT4; Polyclonal Antibody; ID (B3GNT4; UDP-GlcNAc:betaGal beta-1; 3-N-acetylglucosaminyltransferase 4) (APC); 3-N-acetylglucosaminyltransferase 4; anti-B3GNT4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-B3GNT4 antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
B3GNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the Central region of human B3GNT4.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(B3GNT4 Antaibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the B3GNT4 antibody detected the B3GNT4 protein (arrow).)
Western Blot (WB)
(B3GNT4 Antaibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the B3GNT4 antibody detected the B3GNT4 protein (arrow).)
Western Blot (WB)
(B3GNT4 Antaibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the B3GNT4 antibody detected the B3GNT4 protein (arrow).)
Western Blot (WB)
(B3GNT4 Antaibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the B3GNT4 antibody detected the B3GNT4 protein (arrow).)
Flow Cytometry (FC/FACS)
(B3GNT4 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(B3GNT4 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-B3GNT4 antibody
This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase protein family. The encoded enzyme is involved in the biosynthesis of poly-N-acetyllactosamine chains and prefers lacto-N-neotetraose as a substrate. It is a type II transmembrane protein. [provided by RefSeq].
Product Categories/Family for anti-B3GNT4 antibody
References
Shiraishi, N., et al. J. Biol. Chem. 276(5):3498-3507(2001). Amado, M., et al. Biochim. Biophys. Acta 1473(1):35-53(1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,310 Da
NCBI Official Full Name
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4
NCBI Official Synonym Full Names
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4
NCBI Official Symbol
B3GNT4
NCBI Official Synonym Symbols
B3GN-T4; beta3Gn-T4
NCBI Protein Information
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4; BGnT-4; beta-1,3-Gn-T4; beta-1,3-N-acetylglucosaminyltransferase bGn-T4
UniProt Protein Name
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 4
UniProt Gene Name
B3GNT4
UniProt Synonym Gene Names
BGnT-4; Beta-1,3-Gn-T4; Beta-1,3-N-acetylglucosaminyltransferase 4; Beta3Gn-T4
Uniprot Description
Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Has activity for type 2 oligosaccharides.
Similar Products
Product Notes
The B3GNT4 b3gnt4 (Catalog #AAA6277141) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The B3GNT4, ID (B3GNT4, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4) (APC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's B3GNT4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the B3GNT4 b3gnt4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B3GNT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
