Rabbit anti-Human B3GAT3 Polyclonal Antibody | anti-B3GAT3 antibody

B3GAT3 (Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3, Beta-1,3-glucuronyltransferase 3, Glucuronosyltransferase I, UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase) (Biotin)

Cat. Num. AAA6370839

• Gene Names: B3GAT3; GLCATI; glcUAT-I
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: B3GAT3; Polyclonal Antibody; B3GAT3 (Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3; Beta-1; 3-glucuronyltransferase 3; Glucuronosyltransferase I; UDP-GlcUA:Gal beta-1; 3-Gal-R glucuronyltransferase) (Biotin); anti-B3GAT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human B3GAT3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-B3GAT3 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human B3GAT3, aa1-335 (NP_036332.2).
• Immunogen Sequence: MKLKLKNVFLAYFLVSIAGLLYALVQLGQPCDCLPPLRAAAEQLRQKDLRISQLQAELRRPPPAPAQPPEPEALPTIYVVTPTYARLVQKAELVRLSQTLSLVPRLHWLLVEDAEGPTPLVSGLLAASGLLFTHLVVLTPKAQRLREGEPGWVHPRGVEQRNKALDWLRGRGGAVGGEKDPPPPGTQGVVYFADDDNTYSRELFEEMRWTRGVSVWPVGLVGGLRFEGPQVQDGRVVGFHTAWEPSRPFPVDMAGFAVALPLLLDKPNAQFDSTAPRGHLESSLLSHLVDPKDLEPRAANCTRVLVWHTRTEKPKMKQEEQLQRQGRGSDPAIEV
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of B3GAT3 expression in transfected 293T cell line by B3GAT3 polyclonal antibody. Lane 1: B3GAT3 transfected lysate (37.1kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-B3GAT3 antibody

The protein encoded by this gene is a member of the glucuronyltransferase gene family, enzymes that exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product catalyzes the formation of the glycosaminoglycan-protein linkage by way of a glucuronyl transfer reaction in the final step of the biosynthesis of the linkage region of proteoglycans.

Product Categories/Family for anti-B3GAT3 antibody

Antibodies; Enzymes; Glucuronosyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 12408654
• NCBI GeneID: 26229
• NCBI Accession #: NP_036332.2
• NCBI GenBank Nucleotide #: NM_012200.2
• UniProt Accession #: O94766; Q96I06; Q9UEP0
• Molecular Weight: 37,122 Da
• NCBI Official Full Name: galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 isoform 1
• NCBI Official Synonym Full Names: beta-1,3-glucuronyltransferase 3
• NCBI Official Symbol: B3GAT3
• NCBI Official Synonym Symbols: GLCATI; glcUAT-I
• NCBI Protein Information: galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3; Sqv-8-like protein; UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase; beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I)
• UniProt Protein Name: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3
• Protein Family: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase
• UniProt Gene Name: B3GAT3
• UniProt Synonym Gene Names: GlcAT-I; GlcUAT-I
• UniProt Entry Name: B3GA3_HUMAN

Uniprot Description

B3GAT3: Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. Defects in B3GAT3 are the cause of multiple joint dislocations short stature craniofacial dysmorphism and congenital heart defects (JDSSDHD). An autosomal recessive disease characterized by dysmorphic facies, bilateral dislocations of the elbows, hips, and knees, clubfeet, and short stature, as well as cardiovascular defects. Belongs to the glycosyltransferase 43 family.

Protein type: Glycan Metabolism - heparan sulfate biosynthesis; Transferase; EC 2.4.1.135; Membrane protein, integral; Glycan Metabolism - chondroitin sulfate biosynthesis

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: Golgi membrane; Golgi apparatus; cis-Golgi network; membrane; integral to membrane

Molecular Function: protein binding; glucuronosyltransferase activity; metal ion binding; galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity

Biological Process: chondroitin sulfate metabolic process; positive regulation of catalytic activity; glycosaminoglycan biosynthetic process; glycosaminoglycan metabolic process; heparan sulfate proteoglycan biosynthetic process; chondroitin sulfate proteoglycan biosynthetic process; carbohydrate metabolic process; protein amino acid glycosylation; pathogenesis; dermatan sulfate proteoglycan biosynthetic process

Disease: Multiple Joint Dislocations, Short Stature, Craniofacial Dysmorphism, And Congenital Heart Defects