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Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human skin tissue and Human fetal brain tissue lysates, Primary antibody: 824216(B3GALNT2 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 90 seconds)

Rabbit anti-Human B3GALNT2 Polyclonal Antibody | anti-B3GALNT2 antibody

B3GALNT2 Rabbit pAb

Gene Names
B3GALNT2; MDDGA11; B3GalNAc-T2
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
B3GALNT2; Polyclonal Antibody; B3GALNT2 Rabbit pAb; MDDGA11; B3GalNAc-T2; anti-B3GALNT2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-B3GALNT2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
Immunogen
Fusion protein of human B3GALNT2
Conjugation
Unconjugated
Molecular Function
This gene encodes a member of the glycosyltransferase 31 family. The encoded protein synthesizes GalNAc:beta-1, 3GlcNAc, a novel carbohydrate structure, on N- and O-glycans. Alternatively spliced transcript variants that encode different isoforms have been described.
Modification
Unmodified
Cell Position
Endoplasmic reticulum, Golgi apparatus, Membrane
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human skin tissue and Human fetal brain tissue lysates, Primary antibody: 824216(B3GALNT2 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 90 seconds)

Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human skin tissue and Human fetal brain tissue lysates, Primary antibody: 824216(B3GALNT2 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 90 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 824216(B3GALNT2 Antibody) at dilution 1/30. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 824216(B3GALNT2 Antibody) at dilution 1/30. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 824216(B3GALNT2 Antibody) at dilution 1/30. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 824216(B3GALNT2 Antibody) at dilution 1/30. (Original magnification: ×200))
Product Categories/Family for anti-B3GALNT2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
35,649 Da
NCBI Official Full Name
Homo sapiens beta-1,3-N-acetylgalactosaminyltransferase 2, mRNA
NCBI Official Synonym Full Names
beta-1,3-N-acetylgalactosaminyltransferase 2
NCBI Official Symbol
B3GALNT2
NCBI Official Synonym Symbols
MDDGA11; B3GalNAc-T2
NCBI Protein Information
UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 2
UniProt Protein Name
UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 2
UniProt Gene Name
B3GALNT2
UniProt Synonym Gene Names
Beta-1,3-GalNAc-T2
UniProt Entry Name
B3GL2_HUMAN

NCBI Description

This gene encodes a member of the glycosyltransferase 31 family. The encoded protein synthesizes GalNAc:beta-1,3GlcNAc, a novel carbohydrate structure, on N- and O-glycans. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Mar 2013]

Uniprot Description

B3GALNT2: Beta-1,3-N-acetylgalactosaminyltransferase active in synthesizing a unique carbohydrate structure, GalNAc-beta-1- 3GlcNAc, on N- and O-glycans. Has no galactose nor galactosaminyl transferase activity toward any acceptor substrate. Belongs to the glycosyltransferase 31 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.4.1.-; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 1q42.3

Cellular Component: Golgi membrane; endoplasmic reticulum; integral to membrane

Molecular Function: acetylglucosaminyltransferase activity; acetylgalactosaminyltransferase activity; galactosyltransferase activity

Biological Process: protein amino acid O-linked glycosylation; protein amino acid glycosylation

Disease: Muscular Dystrophy-dystroglycanopathy (congenital With Brain And Eye Anomalies), Type A, 11

Research Articles on B3GALNT2

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Product Notes

The B3GALNT2 b3galnt2 (Catalog #AAA4758147) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The B3GALNT2 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's B3GALNT2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000. Researchers should empirically determine the suitability of the B3GALNT2 b3galnt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B3GALNT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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