Rabbit anti-Human b2-Microglobulin Polyclonal Antibody | anti-B2M antibody

b2-Microglobulin, NT (B2M, Beta-2-microglobulin) (MaxLight 650)

Cat. Num. AAA6488138

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: b2-Microglobulin; Polyclonal Antibody; NT (B2M; Beta-2-microglobulin) (MaxLight 650); anti-B2M antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human B2M.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-B2M antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human B2M (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of B2M (arrow) using rabbit polyclonal B2M Antibody (N-term) (RB14679). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the B2M gene (Lane 2) (Origene Technologies).)

Flow Cytometry (FC/FACS)

(B2M Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with B2M antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-B2M antibody

Antibodies; Serum Proteins

References

1. Sakata, M., J. Mol. Biol. 382(5), 1242-1255 (2008). 2. Huang, W.C., Clin. Cancer Res. 14(17), 5341-5347 (2008). 3. Gattoni-Celli, S., Cancer Res. 52(5), 1201-1204 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 4757826
• NCBI GeneID: 567
• NCBI Accession #: NP_004039
• NCBI GenBank Nucleotide #: NM_004048.2
• UniProt Accession #: P61769; P01884; Q540F8; Q6IAT8; Q9UCK0; Q9UD48; Q9UDF4
• Molecular Weight: Predicted: 13 kDa; ; Observed: 12 kDa
• NCBI Official Full Name: beta-2-microglobulin
• NCBI Official Synonym Full Names: beta-2-microglobulin
• NCBI Official Symbol: B2M
• NCBI Protein Information: beta-2-microglobulin; beta-2-microglobin; beta chain of MHC class I molecules
• UniProt Protein Name: Beta-2-microglobulin
• Protein Family: Beta-2-microglobulin
• UniProt Gene Name: B2M
• UniProt Entry Name: B2MG_HUMAN

NCBI Description

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]

Uniprot Description

B2M: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Heterodimer of an alpha chain and a beta chain. Beta-2- microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. Belongs to the beta-2-microglobulin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: Golgi membrane; Golgi apparatus; extracellular space; phagocytic vesicle membrane; focal adhesion; membrane; early endosome membrane; endoplasmic reticulum lumen; cytoplasm; plasma membrane; extracellular region; MHC class I protein complex; external side of plasma membrane

Molecular Function: identical protein binding; protein binding

Biological Process: response to drug; regulation of immune response; viral reproduction; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; T cell differentiation in the thymus; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; regulation of defense response to virus by virus; retinal homeostasis; iron ion homeostasis; antigen processing and presentation of peptide antigen via MHC class I; response to cadmium ion; response to molecule of bacterial origin; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; protein refolding; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent

Disease: Hypoproteinemia, Hypercatabolic