Rabbit anti-Human b2-Microglobulin Polyclonal Antibody | anti-HLA-G antibody

b2-Microglobulin (Biotin) (B2M)

Cat. Num. AAA610793

• Gene Names: HLA-G; MHC-G
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Highly Purified; Puri ed by delipidation, salt fractionation and ion exchange chromatography.
• Synonyms: b2-Microglobulin; Polyclonal Antibody; b2-Microglobulin (Biotin) (B2M); Anti -b2-Microglobulin (Biotin) (B2M); anti-HLA-G antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human beta-2-Microglobulin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, as well as purified and partially purified beta-2-Microglobulin (human urine).
• Purity/Purification: Highly Purified; Puri ed by delipidation, salt fractionation and ion exchange chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 250ul sterile ddH2O or 40-50% glycerol. Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.

• Applicable Applications for anti-HLA-G antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy.; Dilution: Western Blot: 1:1000-1:5000; ELISA: 1:2000-1:10,000; Immunohistochemistry: 1:500-1:3000
• Immunogen: Beta-2-Microglobulin (human urine).
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-HLA-G antibody

Beta-2-microglobulin (B2M) is found in the serum of normal individuals and in the urine in elevated amounts in patients with Wilson disease, cadmium poisoning, and other conditions leading to renal tubular dysfunction. Like immunoglobulins, prealbumin, and the beta protein found in the amyloid of Alzheimer disease, B2M has a predominantly beta-pleated sheet structure that may adopt the fibrillar configuration of amyloid in certain pathologic states. The protein is a single polypeptide chain of molecular weight 11,600 (human 119 aa, chromosome 15). B2M associates with heavy chain of class I MHC antigens on the cell surface. A transient complex of MHC-heavy chain and B2M is is known to be assembled into the TAP molecule involving interaction with a number of chaperones. Binding of the processed peptide releases the class I-B2M complex to the cell surface. Absence of binding leads to degradation in the proteasome. Progressive hepatic iron overload, indistinguishable from that observed in HFE, was found only in mice homozygous for the mutated B2M gene.

Product Categories/Family for anti-HLA-G antibody

Antibodies; Abs to Serum Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 188468
• NCBI GeneID: 3135
• UniProt Accession #: O02923; O02924; O02925; O02926; O02927; O02928; O02929; O19588; O19658; O19659
• Molecular Weight: 31,549 Da
• NCBI Official Full Name: b2 microglobulin
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, G
• NCBI Official Symbol: HLA-G
• NCBI Official Synonym Symbols: MHC-G
• NCBI Protein Information: HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; b2 microglobulin; OTTHUMP00000029218; OTTHUMP00000029220; OTTHUMP00000033527; OTTHUMP00000159507; HLA class I molecule; MHC class I antigen G; HLA-G histocompatibility antigen, class I, G
• UniProt Protein Name: HLA-G protein
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-G
• UniProt Entry Name: O02923_HUMAN

NCBI Description

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq]

Uniprot Description

Sequence similarities: Belongs to the MHC class I family. RuleBase RU004439