Rabbit B-Raf Kinase Polyclonal Antibody | anti-braf antibody
B-Raf Kinase (BRAF, Ras effector)
Gene Names
braf; B-Raf; braf1; rafb1; b-raf1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
B-Raf Kinase; Polyclonal Antibody; B-Raf Kinase (BRAF; Ras effector); Anti -B-Raf Kinase (BRAF; anti-braf antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes full length recombinant human BRaf (plus 6His tag, Mr ~94kD) and B-Raf splice variants in cell lysates (Mr ~68-94kD). Species Crossreactivity: Rat and mouse.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Applicable Applications for anti-braf antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot, MAP Kinase Activation and MBP Phosphorylation assays.
Dilution: Western Blot: 1-2ug/ml detects B-Raf splice variants in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme.
MAP Kinase Activation and MBP Phosphorylation: 4ug
Dilution: Western Blot: 1-2ug/ml detects B-Raf splice variants in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme.
MAP Kinase Activation and MBP Phosphorylation: 4ug
Immunogen
Synthetic peptide corresponding to aa754-765, IQAGGYGAFPVH of human B-Raf, with an N-terminal cysteine added for conjugation to KLH.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-braf antibody
Mutations of the BRAF gene implicated in human cancer. Cancers arise owing to the accumulation of mutations in critical genes that alter normal programmes of cell proliferation, differentiation and death. As the first stage of a systematic genome-wide screen for these genes, Davies et al. (2002, UI:22082291) have prioritized for analysis signaling pathways in which at least one gene is mutated in human cancer. The RAS (CG:176)-RAF (CG:93)-MEK (CG:1316)-ERK (CG:412)-MAP kinase pathway mediates cellular responses to growth signals. Ras is mutated to an oncogenic form in about 15% of human cancer. The three Raf genes code for cytoplasmic serine/threonine kinases that are regulated by binding RAS.
Product Categories/Family for anti-braf antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
B-Raf
NCBI Official Synonym Full Names
v-raf murine sarcoma viral oncogene homolog B1
NCBI Official Symbol
braf
NCBI Official Synonym Symbols
B-Raf; braf1; rafb1; b-raf1
NCBI Protein Information
B-Raf; serine/threonine protein kinase BRAF
Protein Family
Research Articles on braf
Similar Products
Product Notes
The braf (Catalog #AAA612626) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The B-Raf Kinase (BRAF, Ras effector) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's B-Raf Kinase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot, MAP Kinase Activation and MBP Phosphorylation assays. Dilution: Western Blot: 1-2ug/ml detects B-Raf splice variants in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme. MAP Kinase Activation and MBP Phosphorylation: 4ug. Researchers should empirically determine the suitability of the braf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B-Raf Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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