Western Blot (WB)
(Western Blot: Sample: Recombinant BAFF, Mouse.)
Rabbit anti-Mouse B-Cell Activating Factor (BAFF) Polyclonal Antibody | anti-BAFF antibody
Polyclonal Antibody to B-Cell Activating Factor (BAFF)
Gene Names
Tnfsf13b; BAFF; BLyS; TALL1; THANK; zTNF4; TALL-1; Tnlg7a; TNFSF20; D8Ertd387e
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
B-Cell Activating Factor (BAFF); Polyclonal Antibody; Polyclonal Antibody to B-Cell Activating Factor (BAFF); anti-BAFF antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against BAFF. It has been selected for its ability to recognize BAFF in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- YQL AALQADLMNL RMELQSYRGS ATPAAAGAPE LTAGVKLLTP AAPRPHNSSR GHRNRRAFQG PEETEQDVDL SAPPAPCLPG CRHSQHDDNG MNLRNIIQDC LQLIADSDTP TIRKGTYTFV PWLLSFKRGN ALEEKENKIV VRQTGYFFIY SQVLYTDPIF AMGHVIQRKK VHVFGDELSL VTLFRCIQNM PKTLPNNSCY SAGIARLEEG DEIQLAIPRE NAQISRNGDD TF
MGHHHHHHSGSEF- YQL AALQADLMNL RMELQSYRGS ATPAAAGAPE LTAGVKLLTP AAPRPHNSSR GHRNRRAFQG PEETEQDVDL SAPPAPCLPG CRHSQHDDNG MNLRNIIQDC LQLIADSDTP TIRKGTYTFV PWLLSFKRGN ALEEKENKIV VRQTGYFFIY SQVLYTDPIF AMGHVIQRKK VHVFGDELSL VTLFRCIQNM PKTLPNNSCY SAGIARLEEG DEIQLAIPRE NAQISRNGDD TF
Sequence Length
285
Applicable Applications for anti-BAFF antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant BAFF (Tyr68~Phe302) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2057926
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant BAFF, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant BAFF, Mouse.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,166 Da
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 13B isoform 2
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 13b
NCBI Official Symbol
Tnfsf13b
NCBI Official Synonym Symbols
BAFF; BLyS; TALL1; THANK; zTNF4; TALL-1; Tnlg7a; TNFSF20; D8Ertd387e
NCBI Protein Information
tumor necrosis factor ligand superfamily member 13B
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 13B
UniProt Gene Name
Tnfsf13b
UniProt Synonym Gene Names
Baff
Uniprot Description
BAFF: Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis; Cell cycle regulation; Cytokine; Membrane protein, integral
Chromosomal Location of Human Ortholog: 8 A1.1|8 4.55 cM
Cellular Component: cytoplasm; extracellular region; extracellular space; integral component of membrane; membrane; perinuclear region of cytoplasm; plasma membrane
Molecular Function: cytokine activity; tumor necrosis factor receptor binding
Biological Process: B cell costimulation; B cell homeostasis; immune response; immunoglobulin secretion; positive regulation of B cell proliferation; positive regulation of germinal center formation; positive regulation of T cell proliferation; regulation of immune response; T cell costimulation
Research Articles on BAFF
Similar Products
Product Notes
The BAFF tnfsf13b (Catalog #AAA2006987) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to B-Cell Activating Factor (BAFF) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's B-Cell Activating Factor (BAFF) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the BAFF tnfsf13b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- YQL AALQADLMNL RMELQSYRGS ATPAAAGAPE LTAGVKLLTP AAPRPHNSSR GHRNRRAFQG PEETEQDVDL SAPPAPCLPG CRHSQHDDNG MNLRNIIQDC LQLIADSDTP TIRKGTYTFV PWLLSFKRGN ALEEKENKIV VRQTGYFFIY SQVLYTDPIF AMGHVIQRKK VHVFGDELSL VTLFRCIQNM PKTLPNNSCY SAGIARLEEG DEIQLAIPRE NAQISRNGDD TF. It is sometimes possible for the material contained within the vial of "B-Cell Activating Factor (BAFF), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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