Rabbit AXIN2 Polyclonal Antibody | anti-AXIN2 antibody

AXIN2 Antibody

Cat. Num. AAA150049

• Gene Names: AXIN2; AXIL; ODCRCS
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: AXIN2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: AXIN2; Polyclonal Antibody; AXIN2 Antibody; AXIN2 Antibody: AXIL; ODCRCS; Axin-2; Axin-like protein; Axil; anti-AXIN2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: AXIN2 antibody is predicted to not cross-react with AXIN1. At least four isoforms of AXIN2 are known to exist; this antibody will recognize all four.
• Purity/Purification: AXIN2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; AXIN2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 843

• Applicable Applications for anti-AXIN2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
• Application Notes: AXIN2 antibody can be used for detection of AXIN2 by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples.; ; All other applications and species not yet tested.; Optimal dilutions for each application to be determined by the researcher.
• Conjugate: Unconjugated
• Immunogen: AXIN2 antibody was raised against a 20 amino acid synthetic peptide near the carboxyterminus of human AXIN2.; The immunogen is located within amino acids 780 - 830 of AXIN2.
• Homology: Predicted species reactivity based on immunogen sequence: Chicken: (85%)
• Positive Control: MBS151736 Mouse Lung Tissue Lysate
• Preparation and Storage: AXIN2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of AXIN2 in mouse lung lysate with AXIN2 antibody at 1 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of AXIN2 in rat lung tissue with AXIN2 antibody at 5 ug/mL.)

Immunofluorescence (IF)

(Immunofluorescence of AXIN2 in Rat Lung cells with AXIN2antibody at 20 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of AXIN2 in mouse lung tissue with AXIN2 antibody at 5 ug/mL.)

Immunofluorescence (IF)

(Immunofluorescence of AXIN2 in mouse lung tissue with AXIN2 antibody at 20 ug/mL.Red: AXIN2 Antibody (MBS150049) Blue: DAPI staining)

Immunofluorescence (IF)

(Immunofluorescence of AXIN2 in HepG2 cells with AXIN2 Antibody at 20 ug/ml.)

Related Product Information for anti-AXIN2 antibody

AXIN2 Antibody: Like the related protein AXIN1, AXIN2 is thought to play an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway. In mouse, AXIN2 organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and AXIN2, which leads to the degradation of beta-catenin, which is thought to be an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.

NCBI and Uniprot Product Information

• NCBI GI #: 195927059
• NCBI GeneID: 8313
• NCBI Accession #: AAF22799
• NCBI GenBank Nucleotide #: NM_004655.3
• Molecular Weight: 85, 93 kDa
• NCBI Official Full Name: axin-2
• NCBI Official Synonym Full Names: axin 2
• NCBI Official Symbol: AXIN2
• NCBI Official Synonym Symbols: AXIL; ODCRCS
• NCBI Protein Information: axin-2; conductin; axin-like protein; axis inhibition protein 2
• UniProt Protein Name: Axin-2
• Protein Family: Axin
• UniProt Gene Name: AXIN2
• UniProt Synonym Gene Names: Axil
• UniProt Entry Name: AXIN2_HUMAN

NCBI Description

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008]

Uniprot Description

axin 2: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Is downregulated during progression of esophageal squamous cell carcinoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q24.1

Cellular Component: Golgi apparatus; centrosome; cytoplasmic membrane-bound vesicle; postsynaptic density; cell cortex; cytosol; beta-catenin destruction complex; nucleoplasm; microtubule cytoskeleton; cytoplasmic microtubule; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; beta-catenin binding; protein kinase binding; GTPase activator activity

Biological Process: odontogenesis; cell proliferation; negative regulation of cell proliferation; intramembranous ossification; regulation of mismatch repair; mRNA stabilization; negative regulation of osteoblast differentiation; dorsal/ventral axis specification; positive regulation of protein amino acid phosphorylation; maintenance of DNA repeat elements; bone mineralization; positive regulation of GTPase activity

Disease: Oligodontia-colorectal Cancer Syndrome