Rabbit anti-Human AXIN2 Polyclonal Antibody | anti-AXIN2 antibody

AXIN2, CT (AXIN2, Axin-2, Axin-like protein, Axis inhibition protein 2, Conductin) (AP)

Cat. Num. AAA6277019

• Gene Names: AXIN2; AXIL; ODCRCS
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AXIN2; Polyclonal Antibody; CT (AXIN2; Axin-2; Axin-like protein; Axis inhibition protein 2; Conductin) (AP); Conductin; anti-AXIN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AXIN2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-AXIN2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 816-843 in the C-terminal region of human AXIN2, UniProt Accession #Q9Y2T1. Species sequence homology: mouse, rat
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS6007616. This demonstrates the AXIN2 antibody detected the AXIN2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS6007616 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6007616 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells (right histogram) using MBS6007616 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-AXIN2 antibody

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.

Product Categories/Family for anti-AXIN2 antibody

Antibodies; Wnt Proteins

References

1. Inkster, B., et al. Neuroimage (2010). In press, 2. Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010), 3. Couch, F.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(1):251-257(2010), 4. Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870 (2009), 5. Olschwang, S., et al. J Oncol 2009, 306786 (2009), 5. Dong, X., et al. Cytogenet. Cell Genet. 93 (1-2),:26-28 (2001), 7. Liu, W., et al. Nat. Genet. 26(2):146-147(2000), 8. von Kries, J.P., et al. Nat. Struct. Biol. 7(9):800-807(2000), 9. Kikuchi, A. Cytokine Growth Factor Rev. 10 (3-4):255-265 (1999), 10. Mai, M., et al. Genomics 55(3):341-344(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 195927059
• NCBI GeneID: 8313
• NCBI Accession #: NP_004646.3
• NCBI GenBank Nucleotide #: NM_004655.3
• UniProt Accession #: Q9Y2T1; Q3MJ88; Q9H3M6; Q9UH84
• Molecular Weight: 93,558 Da
• NCBI Official Full Name: axin-2
• NCBI Official Synonym Full Names: axin 2
• NCBI Official Symbol: AXIN2
• NCBI Official Synonym Symbols: AXIL; ODCRCS
• NCBI Protein Information: axin-2; conductin; axin-like protein; axis inhibition protein 2
• UniProt Protein Name: Axin-2
• Protein Family: Axin
• UniProt Gene Name: AXIN2
• UniProt Synonym Gene Names: Axil
• UniProt Entry Name: AXIN2_HUMAN

NCBI Description

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008]

Uniprot Description

axin 2: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Is downregulated during progression of esophageal squamous cell carcinoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q24.1

Cellular Component: microtubule cytoskeleton; nucleoplasm; Golgi apparatus; centrosome; cytoplasmic microtubule; postsynaptic density; cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; cell cortex; cytosol; nucleus; beta-catenin destruction complex

Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; beta-catenin binding; protein kinase binding; GTPase activator activity

Biological Process: odontogenesis; negative regulation of cell proliferation; cell proliferation; intramembranous ossification; regulation of mismatch repair; mRNA stabilization; negative regulation of osteoblast differentiation; dorsal/ventral axis specification; positive regulation of protein amino acid phosphorylation; maintenance of DNA repeat elements; bone mineralization; positive regulation of GTPase activity

Disease: Oligodontia-colorectal Cancer Syndrome