Rabbit Aven Polyclonal Antibody | anti-Aven antibody

Aven (P329) Polyclonal Antibody

Cat. Num. AAA3002305

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Aven; Polyclonal Antibody; Aven (P329) Polyclonal Antibody; Myosin-15; Myosin heavy chain 15; MYH15; KIAA1000; anti-Aven antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Aven pAb detects endogenous levels of Aven protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 362

• Applicable Applications for anti-Aven antibody: Western Blot (WB)
• Application Notes: WB: 1:1000-1:2000
• Immunogen: Synthetic peptide, corresponding Human MYH15.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Aven (P329) pAb at 1:1000 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:L02 whole cell lysate (40ug) Lane3:The Testis tissue lysate of Mouse (40ug) Lane4:The Heart tissue lysate of Rat (40ug))

Related Product Information for anti-Aven antibody

The Bcl-2 family regulates apoptosis in response to a wide range of stimuli through control of mitochondrial cytochrome c release and caspase activation. Cytosolic Apaf-1 forms a complex with caspase-9 in the presence of cytochrome c and dATP, ultimately leading to caspase-9 activation and subsequent activation of caspase-3. A large number of proteins have been found to interact with Bcl-2 and other family members that have been shown to help regulate apoptosis. Aven was identified in a yeast two-hybrid screen as a bcl-xL interacting protein. It also interacts with other anti-apoptotic family members, including Bcl-2, but fails to interact with pro-apopotic proteins Bax and Bak. Aven inhibits apoptosis and enhances anti-apopotic activity of Bcl-xL. It interferes with association with Apaf-1 and activation of caspase-9. Aven overexpression is associated with poor prognosis in acute lymphoblastic leukemia.

NCBI and Uniprot Product Information

• NCBI GI #: 9966841
• NCBI Accession #: NP_065104.1
• NCBI GenBank Nucleotide #: NP_065104.1
• UniProt Accession #: Q9NQS1
• Molecular Weight: ~ 50kDa
• NCBI Official Full Name: cell death regulator Aven
• UniProt Protein Name: Cell death regulator Aven
• Protein Family: Avenin
• UniProt Gene Name: AVEN
• UniProt Entry Name: AVEN_HUMAN

Uniprot Description

AVEN: Protects against apoptosis mediated by Apaf-1. Binds Apaf-1, BCL-2 and BAD (Bcl-xl). Highly expressed in testis, ovary, thymus, prostate, spleen, small intestine, colon, heart, skeletal muscle, liver, kidney and pancreas.

Protein type: Inhibitor; Apoptosis

Chromosomal Location of Human Ortholog: 15q13.1

Cellular Component: membrane; endomembrane system; intracellular

Molecular Function: protein binding

Biological Process: apoptosis; negative regulation of apoptosis