Rabbit anti-Human, Mouse ATRX Polyclonal Antibody | anti-ATRX antibody

ATRX Antibody

Cat. Num. AAA9410230

• Gene Names: ATRX; JMS; SHS; XH2; XNP; ATR2; SFM1; MRX52; RAD54; MRXHF1; RAD54L; ZNF-HX
• Reactivity: Human, Mouse
• Applications: Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: ATRX; Polyclonal Antibody; ATRX Antibody; EC 3.6.1.-; RAD54L; Transcriptional regulator ATRX; X-linked helicase II; X-linked nuclear protein; anti-ATRX antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total ATRX protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 2492

• Applicable Applications for anti-ATRX antibody: Immunofluorescence (IF)
• Application Notes: Immunofluorescence: 1:100~1:500
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from N-terminal of human ATRX.
• Target Name: ATRX
• Preparation and Storage: Store at -20 degree C

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cells, using ATRX antibody.)

Related Product Information for anti-ATRX antibody

Involved in transcriptional regulation and chromatin remodeling. Facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. Binds to DNA tandem repeat sequences in both telomeres and euchromatin and in vitro binds DNA quadruplex structures. May help stabilizing G-rich regions into regular chromatin structures by remodeling G4 DNA and incorporating H3.3-containing nucleosomes. Catalytic component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Its heterochromatin targeting is proposed to involve a combinatorial readout of histone H3 modifications (specifically methylation states of H3K9 and H3K4) and association with CBX5. Involved in maintaining telomere structural integrity in embryonic stem cells which probably implies recruitment of CBX5 to telomers. Reports on the involvement in transcriptional regulation of telomeric repeat-containing RNA (TERRA) are conflicting; according () is not sufficient to decrease chromatin condensation at telomers nor to increase expression of telomeric RNA in fibroblasts. May be involved in telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines. Acts as negative regulator of chromatin incorporation of transcriptionally repressive histone H2AFY, particularily at telomeres and the alpha-globin cluster in erythroleukemic cells. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, required for the chromatin occupancy of SMC1 and CTCTF within the H19 imprinting control region (ICR) and involved in esatblishment of histone tails modifications in the ICR. May be involved in brain development and facial morphogenesis.Picketts D.J., Hum. Mol. Genet. 5:1899-1907(1996).Villard L., Genomics 43:149-155(1997).Kitano T., Mol. Biol. Evol. 20:1281-1289(2003).

Product Categories/Family for anti-ATRX antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 530788276
• NCBI GeneID: 546
• NCBI Accession #: NP_000480.3
• NCBI GenBank Nucleotide #: NM_000489.4
• UniProt Accession #: P46100; P51068; Q15886; Q59FB5; Q59H31; Q5H9A2; Q5JWI4; Q7Z2J1; Q9H0Z1; Q9NTS3; D3DTE2
• Molecular Weight: 151,556 Da
• NCBI Official Full Name: transcriptional regulator ATRX isoform 1
• NCBI Official Synonym Full Names: alpha thalassemia/mental retardation syndrome X-linked
• NCBI Official Symbol: ATRX
• NCBI Official Synonym Symbols: JMS; SHS; XH2; XNP; ATR2; SFM1; MRX52; RAD54; MRXHF1; RAD54L; ZNF-HX
• NCBI Protein Information: transcriptional regulator ATRX
• UniProt Protein Name: Transcriptional regulator ATRX
• Protein Family: Transcriptional regulator
• UniProt Gene Name: ATRX
• UniProt Synonym Gene Names: RAD54L; XH2; XNP
• UniProt Entry Name: ATRX_HUMAN

NCBI Description

The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Mutations in this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Aug 2013]

Uniprot Description

ATRX: a global transcriptional regulator. Belongs to the SNF2 family of proteins, many of which modify gene expression via chromatin remodeling activity. Involved in the developmental silencing of imprinted genes in the brain. Contains one PxVxL motif, which is required for interaction with chromoshadow domains. This motif requires additional residues at -7, -6, +4 and +5 relative to the central V which contact the chromoshadow domain. Constitutive mutations in ATRX are associated with brain, facial, and genital abnormalities, and alpha thalassemia. Acquired mutations in ATRX have been observed in preleukemic conditions. Six alternatively spliced human isoforms have been described.

Protein type: EC 3.6.4.12; Helicase; Ubiquitin conjugating system; DNA repair, damage

Chromosomal Location of Human Ortholog: Xq21.1

Cellular Component: nucleoplasm; PML body; nuclear heterochromatin; nucleus

Molecular Function: protein binding; DNA helicase activity; DNA binding; zinc ion binding; histone binding; chromatin binding; helicase activity; DNA translocase activity; methylated histone residue binding; ATP binding

Biological Process: DNA damage response, signal transduction by p53 class mediator; transcription, DNA-dependent; positive regulation of telomere maintenance; DNA repair; Sertoli cell development; DNA duplex unwinding; replication fork processing; DNA recombination; chromatin remodeling; nucleosome assembly; DNA replication-independent nucleosome assembly; regulation of transcription, DNA-dependent; forebrain development; DNA methylation; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis

Disease: Mental Retardation-hypotonic Facies Syndrome, X-linked, 1; Alpha-thalassemia/mental Retardation Syndrome, X-linked; Alpha-thalassemia Myelodysplasia Syndrome