Human E3 ubiquitin-protein ligase HACE1 ELISA Kit | HACE1 elisa kit

Human E3 ubiquitin-protein ligase HACE1 ELISA Kit

Cat. Num. AAA764362

• Gene Names: HACE1; SPPRS
• Reactivity: Human
• Synonyms: E3 ubiquitin-protein ligase HACE1; Human E3 ubiquitin-protein ligase HACE1 ELISA Kit; HACE1/HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1/HACE1/KIAA1320/6.3.2.-; HACE1 elisa kit

• Reactivity: Human
• Sequence Length: 909

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for HACE1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human HACE1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HACE1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HACE1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman HACE1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 116875852
• NCBI GeneID: 57531
• NCBI Accession #: NP_065822.2
• NCBI GenBank Nucleotide #: NM_020771.3
• Molecular Weight: 77,963 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase HACE1
• NCBI Official Synonym Full Names: HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1
• NCBI Official Symbol: HACE1
• NCBI Official Synonym Symbols: SPPRS
• NCBI Protein Information: E3 ubiquitin-protein ligase HACE1
• UniProt Protein Name: E3 ubiquitin-protein ligase HACE1
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: HACE1
• UniProt Synonym Gene Names: KIAA1320
• UniProt Entry Name: HACE1_HUMAN

Uniprot Description

HACE1: E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB. Defects in HACE1 are a cause of Wilms tumor (WT). WT is a pediatric malignancy of kidney and one of the most common solid cancers in childhood. HACE1 is epigenetically down-regulated in sporadic Wilms tumor. Moreover, a t(5;6)(q21;q21) translocation that truncates HACE1 has been found in a child with bilateral, young-onset Wilms tumor. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; Ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 6q16.3

Cellular Component: endoplasmic reticulum; Golgi membrane

Molecular Function: ligase activity; protein binding; Rab GTPase binding; Rac GTPase binding; ubiquitin-protein ligase activity

Biological Process: cell cycle; Golgi organization and biogenesis; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; Rac protein signal transduction; regulation of cell migration; regulation of transcription, DNA-dependent; transcription, DNA-dependent