Rabbit anti-Human ATRIP Polyclonal Antibody | anti-ATRIP antibody

ATRIP Antibody

Cat. Num. AAA1499757

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: ATRIP; Polyclonal Antibody; ATRIP Antibody; ATM and Rad3-related-interacting protein; AGS1; anti-ATRIP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 664

• Applicable Applications for anti-ATRIP antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:200-1000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human ATR-interacting protein (1-260aa)
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: ATRIP antibody at 8ug/ml+PC-3 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 86,84,77,73 kDaObserved band size: 86 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human ovary using MBS1499757 at dilution 1:100)

Related Product Information for anti-ATRIP antibody

Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.

NCBI and Uniprot Product Information

• NCBI GI #: 402534571
• NCBI GeneID: 84126
• NCBI Accession #: NP_001257951.1
• NCBI GenBank Nucleotide #: NM_001271022.1
• UniProt Accession #: Q8WXE1; Q69YK9; Q8NHQ2; Q8WUG7; Q96CL3; Q9HA30; A8K6A3; A8K714; B2RCE7; B4DU92; B5MEB7
• Molecular Weight: 72,362 Da
• NCBI Official Full Name: ATR-interacting protein isoform 3
• NCBI Official Synonym Full Names: ATR interacting protein
• NCBI Official Symbol: ATRIP
• NCBI Protein Information: ATR-interacting protein
• UniProt Protein Name: ATR-interacting protein
• Protein Family: ATR-interacting protein
• UniProt Gene Name: ATRIP
• UniProt Synonym Gene Names: AGS1
• UniProt Entry Name: ATRIP_HUMAN

NCBI Description

This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2012]

Uniprot Description

ATRIP: is required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein. Heterodimerizes with ATR. The heterodimer binds replication protein A (RPA), a protein complex that subsequently is recruited to single stranded DNA (ssDNA). Its EEXXXDDL motif is required for the interaction with catalytic subunit of DNA-PK and its recruitment to sites of DNA damage. e for this protein is either identical to or adjacent to that of ATRIP. Some of the mRNAs that encode ATRIP also encode TREX1 in another reading frame. Three alternatively spliced human isoforms have been reported.

Protein type: DNA repair, damage; DNA-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: nucleoplasm

Molecular Function: protein binding

Biological Process: DNA damage checkpoint; DNA repair; DNA replication; double-strand break repair; double-strand break repair via homologous recombination