Rabbit atpE Polyclonal Antibody | anti-atpE antibody

Rabbit anti-Shigella flexneri atpE Polyclonal Antibody

• Reactivity: Shigella flexneri
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: atpE; Polyclonal Antibody; Rabbit anti-Shigella flexneri atpE Polyclonal Antibody; atpE Antibody; ATP synthase subunit c; ATP synthase F(0; sector subunit c; F-type ATPase subunit c; F-ATPase subunit c; Lipid-binding protein; atpE SF3817 S3951; anti-atpE antibody

• Host: Rabbit
• Reactivity: Shigella flexneri
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-atpE antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Shigella flexneri atpE protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 24115040
• NCBI GeneID: 1026094
• NCBI Accession #: NP_709550.1
• NCBI GenBank Nucleotide #: NC_004337.2
• UniProt Accession #: P68705; P00844
• Molecular Weight: 8,256 Da
• NCBI Official Full Name: ATP synthase F0F1 subunit C
• NCBI Official Symbol: atpE
• NCBI Protein Information: ATP synthase F0F1 subunit C
• UniProt Protein Name: ATP synthase subunit c
• Protein Family: ATP synthase
• UniProt Gene Name: atpE
• UniProt Synonym Gene Names: F-ATPase subunit c
• UniProt Entry Name: ATPL_SHIFL

Uniprot Description

Function: F1F0 ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F1 containing the extramembraneous catalytic core and F0 containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation

By similarity. HAMAP-Rule MF_01396Key component of the F0 channel; it plays a direct role in translocation across the membrane. A homomeric c-ring of between 10-14 subunits forms the central stalk rotor element with the F1 delta and epsilon subunits

By similarity. HAMAP-Rule MF_01396

Subunit structure: F-type ATPases have 2 components, F1 - the catalytic core - and F0 - the membrane proton channel. F1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. F0 has three main subunits: a1, b2 and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F1 is attached to F0 by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains

By similarity.

Subcellular location: Cell inner membrane; Multi-pass membrane protein

By similarity HAMAP-Rule MF_01396.

Miscellaneous: Dicyclohexylcarbodiimide (DCDD) binding to the active aspartate residue inhibits ATPase in vitro

By similarity. HAMAP-Rule MF_01396

Sequence similarities: Belongs to the ATPase C chain family.